Fatty acids, C16-18 and C18-unsatd., Me esters, distn. residues

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From March 07,2010 to March 09,2010

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant study conducted to recognized international test guidelines; some lacking in some observation and method description.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Fatty acids, C6-24 and C6-24-unsatd., Me esters, distn. residues- Physical state: black , brown semisolid- Analytical purity:100% - Storage condition of test material: room temperature- Solubility: < 10% in water, soluble in acetone, hexane and dichloromethane

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method:Direct weight of the test substance into the test vessels, filled up to final volumes of 10 L with copper- and chlorine-free drinking water, then stirring 5 minutes by ultraturrax.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM- Common name:Leuciscus idus- Length at study initiation : 6 cm long- Feeding during test : no- number of test organism : 10 per concentration- Light : about 16 hours illumination per day- Test vessels 1 fish basins containing 10 L test water

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

The fish did not show any abnormal behaviour.

Hardness:

about 16 °dH (about 93 mg Ca and 12 nig Mg per litre)

Test temperature:

19.5 - 20.5 °C

pH:

7.9-8.3

Dissolved oxygen:

42-100 %

Salinity:

no data

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

ca. 10 000 other: mg product / L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 other: mg product / L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 other: mg product / L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

Fatty acids, C6-24 and C6-24-unsatd., Me esters, distn. Residues turned out to be not toxic to Golden orfes.

Executive summary:

The acute toxicity of Fatty acids, C6 -C24 and C6-24-unsatd., Me esters, distn. Residues to Golden orfes was determined according to the German standard method for the examination of water, waste water and sludge; bioassays (group L); determination of the effect of substances in water on fish - fish-test (L 15). This method corresponds to the OECD-Guideline 203. The product was tested in a concentration range of 1 to 10000 mg product/ 1 (nominal). The incubation period was 48 hours under static conditions (the test-solutions were not renewed).

Description of key information

LC0= 10000 mg/L

Additional information

Fatty acids, C16 -18 and C18-unsatd., Me esters, distn. Residues are considered to be not toxic to fish