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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

LC50 (96 h) Zebra fish > 100 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

The acute toxicity of the substance under registration on the fish Oncorhynchus mykiss was investigated over a period of 48 h, with the Modified Bioassay Method (n. a., 1976). Based on mortality data at 48 h of exposure the LC50 of the test item resulted higher than 1000 mg/l. The study is only a summary and the test duration was of 48 h instead than 96 h, therefore the study was considered as supporting and studies on similar substances belonging to the Stilbene Fluorescent Whitening Agents category were reported. Justification for Read Across is given in Section 13 of IUCLID.


Testing results on the similar substance 3a-A(NaK) were considered. The similar substance has very similar chemical structure to 5 -A. Acute toxicity to Zebra fish was evaluated in the 96 hours screening test, according to the OECD guideline 203. A single dose was administrated at 100 mg/l (limit test) with no effect (Novartis Services AG., 1999).

Two fish acute toxicity tests are available (Ciba-Geigy, 1994 and 1998) on the similar substance 2 -A pre-treated with light in order to obtain a high percentage of photodegraded products and verify their impact on aquatic toxicity. Both tests resulted in a LC50 > 100 mg/l, demonstrating that those degradation products are no more dangerous for the environment than the parent compounds.

In conclusion, no fish acute toxicity is expected for the substance.