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EC number: 248-420-5 | CAS number: 27344-06-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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- Acute Toxicity
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The test procedure cannot be subsumed under a testing guideline, nevertheless are well documented and scientifically acceptable. The test was not performed on GLP. The OECD recommended combination of strains was tested.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
- Principles of method if other than guideline:
- The test was to be carried out for the purpose of checking the back mutation from Histidine requisite to non-requisite in Salmonella Tryphimurium and back mutation from Tryptophane requisite to non-requisite in Caliform Bacilli.
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- Disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- EC Number:
- 248-420-5
- EC Name:
- Disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- Cas Number:
- 27344-06-5
- Molecular formula:
- C42H46N14O10S2.2Na
- IUPAC Name:
- disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: Histidine requisite strain.
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- other: Histidine requisite strain.
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- other: Tryptophane requisite strain.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 additive
- Test concentrations with justification for top dose:
- 5000, 1000, 500, 100, 50 and 10 µg/plate.
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: AF2, 4-nitro-o-phenylenediamine and 2-aminoanthracene.
- Remarks:
- - S9: TA98, TA 100 and WP2 uvrA AF2; TA 1537 9-AC, TA 1538 4-NOPD; TA 1535 ENNG. + S9: 2-AA all strains
- Details on test system and experimental conditions:
- PREPARATION OF SPECIMEN BACILLI
The specimen bacilli solution was made by supplementing 0.5 % NaCl, inoculating specimen bacilli into L-form test tube which contains nutrient broth (Difco), and cultivating them with two-way 16 hour shaking at the temperature of 37 °C.
TEST OPERATION
Plate Incorporation Method.
In the small tube, the specimen bacill solution 0.1 ml , specimen solution 0. 1 ml and phosphate-buffered solution 0.5 ml (S-mixed solution) 0.5ml for the metabolic activation test) were well mixed with 2 ml top agar and the mixture was put on the base agar plate to harden it.
The culture was to be made for 48 hours at 37 °C, and the generating mutation colony was counted.
REAGENTS
1) Top Agar
2) S-9 mixed solution
3) base agar
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- All six strains (Salmonella Typhinurium TA 1535, TA 100, TA 1537, TA 1538, TA 98; "Escherichia coll WP2 uvrA with the dose of 10 - 5000 µg both in the Direct and the Metabolic Activation Tests, remarkable increase in the mutation colony was not admitted.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test item is not judged to possess mutationality concerning the testings conducted with it. - Executive summary:
Method
The test item was evaluated for mutagenic potential in assays for gene mutation, without and with metabolic activation, in five tester strains of Salmonella thimurium and Escherichia coli WP2 avr A at the concentration of 5,000, 1,000, 500, 100, 50 and 10 µg/plate.
The test was to be carried out for the purpose of checking the back mutation from Histidine requisite to non-requisite in Salmonella Tryphimurium and back mutation from Tryptophane requisite to non-requisite in Caliform Bacilli.
Results
In all the strains tested (Salmonella Typhinurium TA 1535, TA 100, TA 1537, TA 1538, TA 98; Escherichia coll WP2 uv rA) with the dose of 10 - 5000 µg both in the Direct and the Metabolic Activation Tests, remarkable increase in the mutation colony was not admitted.
Conclusion
The test item is not judged to possess mutationality concerning the testings conducted with it.
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