Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-07-26 to 1988-10-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study performed in compliance with GLP, available as unpublished report, no restrictions, fully adequate for assessment.

Qualifier:

according to guideline

Guideline:

other: Directive 84/449/EEC, Part C, C.1

Deviations:

no

Principles of method if other than guideline:

Directive 84/449/EEC was replaced by Directive 92/69/EEC and REGULATION (EC) No 440/2008
Equivalent to OECD 203

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Tetrabenzylthiuramdisulphide (TBeTD; same as TBzTD)
- Substance type: Mono-constituent substance
- Physical state: White powder
- Stability under test conditions: TBzTD is stable from pH 3 to 13. Results of test for ready biodegradability: TBzTD showed no biodegradation in a screening test for ready biodegradability. The solution was renewed after 48 hours. TBzTD is not biodegradable in this period of time.
- Storage condition of test material: 20°C
- Analytical purity: > 95%, technically pure
- Purity test date: > 95%, technically pure
- Lot/batch No.: 880526/5 (OR2)

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Aceton was used for the dispersion of the test substance in water at a concentration of equal or less than 1 ml per 1000 ml water.

TBzTD has got an extremely low solubility in water in the order of 10 to 100 µg /l. The fish were exposed to oversaturated dispersions of TBzTD in the test medium at all concentrations tested. TBzTD particles stack to the water surface of the test medium and settled on the bottom of the test vessel. The concentration of TBzTD in the test medium remained constant.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Guppy
- Strain:
- Source: Produced in the Akzo Research Laboratories Arnhem, dept. CRL according to Standard Operation Procedures SOP CRL T9 & 42.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): Approximately 2 cm.
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Feeding during test: No
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

4 d

Post exposure observation period:

none

Hardness:

9 mg Ca/l, 25 mg Mg/l, 7° dH

Test temperature:

23.5 to 24.5°C

pH:

8.2

Nominal and measured concentrations:

Nominal

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 1 liter
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): after 48h
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.6 g biomass/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural water from the aquarium in which the fish were kept. This aquarium was purified by a biological filtration system {including a mechanical filter, an aerobic sprinkling .filter and an anaerobic nitrate removal (Minireef System)
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 15 hours per day
- Light intensity: ambient light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.32, 1.0, 3.2, 10, 32 mg/l
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: All fish survived in a dispersion of 32 mg/l during 96 hours, and no other effects were observed

Sublethal observations / clinical signs:

Concentration (nominal) / mg/l	Test duration / hours
	0	24	48	72	96
	Number of animals surviving
Control (a)	10	10	10	10	10
Control (b)	10	10	10	10	10
Solvent control (a)	10	10	10	10	10
Solvent control (b)	10	10	10	10	10
0.32 (a)	10	10	10	10	10
0.32 (b)	10	10	10	10	10
1.0 (a)	10	10	10	10	10
1.0 (b)	10	10	10	10	10
3.2 (a)	10	10	10	10	10
3.2 (b)	10	10	10	10	10
10 (a)	10	10	10	10	10
10 (b)	10	10	10	10	10
32 (a)	10	10	10	10	10
32 (b)	10	10	10	10	10

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of tetrakis(phenylmethyl)thioperoxydi(carbothioamide) (TBzTD) was carried out under semi-static conditions according to a method equivalent to OECD 201 (Fish, Acute Toxicity Test) with guppy (Poecilia reticulata). The fish survived in a dispersion of 32 mg TBzTD/l during 4 days and showed no deviations in behavior or appearance. The LC50 is higher than the maximum concentration of 32 mg/l.

Executive summary:

The acute toxicity of tetrakis(phenylmethyl)thioperoxydi(carbothioamide) (TBzTD) was carried out under semi-static conditions and concentrations ranging from 0.32 to 32 mg/l according to the Directive 84/449/EECpart C: Methods for the determination of ecotoxicity, C.l. Acute toxicity to fish which is equivalent to OECD 201 (Fish, Acute Toxicity Test)with guppy (Poecilia reticulata). Due to the extremely low solubility of TBzTD it formed a dispersion in water. The fish survived in a dispersion of 32 mg TBzTD/l during 4 days and showed no deviations in behavior or appearance. An LC50 could not be established. It is higher than the maximum concentration of 32 mg/l.

Description of key information

The acute toxicity of tetrakis(phenylmethyl)thioperoxydi(carbothioamide) (TBzTD) was carried out under semi-static conditions according to a method equivalent to OECD 201 (Fish, Acute Toxicity Test) with guppy (Poecilia reticulata). The fish survived in a dispersion of 32 mg TBzTD/l during 4 days and showed no deviations in behavior or appearance. The LC50 is higher than the maximum concentration of 32 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

32 mg/L

Additional information

The acute toxicity of tetrakis(phenylmethyl)thioperoxydi(carbothioamide) (TBzTD) was carried out under semi-static conditions and concentrations ranging from 0.32 to 32 mg/l according to the Directive 84/449/EECpart C: Methods for the determination of ecotoxicity, C.l. Acute toxicity to fish which is equivalent to OECD 201 (Fish, Acute Toxicity Test)with guppy (Poecilia reticulata). Due to the extremely low solubility of TBzTD it formed a dispersion in water. The fish survived in a dispersion of 32 mg TBzTD/l during 4 days and showed no deviations in behavior or appearance. An LC50 could not be established. It is higher than the maximum concentration of 32 mg/l.