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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Monitoring of algae media was performed with DOC. No substance specific analysis was performed.
according to guideline
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
Analytical monitoring:
Details on test solutions:
To accelerate the solution procedure a five fold amount (45 mg/1) of the maximum water solubility of the test substance in the
preliminary test (9 mg/1) was weighed into water, treated for 60 seconds at 8000 rpm.with an ultra-turrax and afterwards stirred
an a magnetic stirrer for 24 hours. To remove undissolved particles, the resulting emulsion was filtered using a folded filter of the
pore size 7-12 gm.
Test organisms (species):
other: Daphnia magna STRAUSS
Details on test organisms:
- Parthenogenetic females, age 0 – 24 hours, laboratory bred at BAYER AG Leverkusen, cloned at the Federal Health Office (BGA), Berlin Germany
- Water for aquaculture: M4-medium according to Elendt, version of BGA (1992), originally described in Water Research 24 (9), Sept. 1990; 1157-1167

Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
14.2 °dH (253.47 mg/l CaCO3)

Test temperature:
20.2 °C (after 48 hrs)

7.9 (after 48 hrs)

Dissolved oxygen:
8.5 mg/l (after 48 hrs)

No data
Nominal and measured concentrations:
Nominal concentration of test substance: 9 mg/l (Analytical value after 0 hour: 5 mg/l, after 48 hours: 6 mg/l, resp.)
Details on test conditions:
- water for dilution: M4 -Medium according to Elendt and BGA (1992)
- control: M4 -Medium according to Elendt and BGA (1992)
- photoperiod: 16 hours light, 8 hours dark (illumination 1000 lux)
- cylindric test vessel: diameter 4.0 cm/height 6.5 cm, 20 ml test medium,not ventilated
- number of daphnids per beaker: 10 daphnids (neonates)
- test parameter: immobilization (death)
- method of administration: direct weighing
- test concentration: 1 (9 mg/l) plus 1 control
- replicate: 2 (per concentration/control)
- chemical analysis: TOC
48 h
Dose descriptor:
Effect conc.:
>= 8.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
other: determined as TOC and back-calculated to the parent compound
Basis for effect:
Remarks on result:
other: Arithmetic mean of analytical values
24 h
Dose descriptor:
Effect conc.:
>= 9 mg/L
Nominal / measured:
Conc. based on:
other: determined as TOC and back-calculated to the parent compound
Basis for effect:
Details on results:
The above test result refers to a concentration of the test substance which was calculated directly from analytically determined TOC values.
Validity criteria fulfilled:
Executive summary:

The acute toxicity of 4,4-methylenedicyclohexyl diisocyanate to Daphnia magna is EC 0 >= 8.3 mg/l at 48 h (Bayer 2000). The result is expressed in terms of arithmetic mean of analytical values. The study was conducted according to the Council Directive 92/69/EEC C.2 (1992).

Description of key information

With the invertebrate Daphnia magna one acute static limit test in accordance with Directive 92/69/EEC, C.2, is available. For a test period of 48 hours an EC50 value of > 8.3 mg/l was determined. At this concentration no mortality was observed (Bayer AG, 2000c).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
Effect concentration:
8.3 mg/L

Additional information

Should read >8.3 mg/L