2,2,4-trimethylpentane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment. Vapour generation and analysis not well documented.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

- limit test concentration: 5 mg/L

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 870.1300 (Acute inhalation toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,4-trimethylpentane

EC Number:

208-759-1

EC Name:

2,2,4-trimethylpentane

Cas Number:

540-84-1

Molecular formula:

C8H18

IUPAC Name:

2,2,4-trimethylpentane

Details on test material:

- Name of test material (as cited in study report): Isooctane
- Analytical purity: 100% pure commercial product

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Duration of exposure:

4 h

Concentrations:

33.52 mg/L nominal concentration
21561.5 ± 776.32 ppm mean analytical concentration

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: day 2, 3, and 4 mentioned, not further specified
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

not reported

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 33.52 mg/L air (nominal)

Exp. duration:

4 h

Mortality:

No mortality occurred during the 14-day observattion period.

Clinical signs:

other: Exposure-related observations noted in all animals during the exposure period included lying prostrate in the cage and rapid respiration. All animals appeared normal throughout the post exposure period.

Body weight:

Sightly decreased body weights were noted in the males on day 2 post exposure and in the females on days 2, 3 and 4 post exposure.

Gross pathology:

No abnormal gross pathology observations were noted in any animals upon necropsy.

Any other information on results incl. tables

Inhalation LC50 > 33.52 mg/L air (nominal) for 4 hours in male and female rats.

Applicant's summary and conclusion

Interpretation of results:

other: not classified

Remarks:

Criteria used for interpretation of results: other: GHS, EU, 2007

Conclusions:

Based on the study design the test substance, 2,2,4-trimethylpentane, needs not to be classified.

Executive summary:

Based on the study design the test substance, 2,2,4 -trimethylpentane needs not to be classified.