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Reliable acute studies based on international guidelines or on calculation methods are available for the three aquatic trophic levels (fish, algae and aquatic invertebrates) to assess the toxicity of test substance Reaction mass of beta-phellandrene and d-limonene and l-limonene.

Among the data available, a toxic effect was observed for these three aquatic trophic levels. The most sensitive species appears to be aquatic invertebrate daphnia magna, with a 48-h EC50 value estimated between 1.7 and 2.3 mg/L (loading) under experimental conditions, according to a reliable experimental study.

Moreover, based on calculation methods, the 96-h LL50 for Reaction mass of β-phellandrene and d-limonene and l-limonene to the fish Danio rerio is 4.1 mg/L and the 72-h ErL50 to the algae P. subcapitata is 3.3 mg/L. For these calculations, the algorithms used were based on QSAR models which have been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004).

Reliable acute studies based on international guidelines or on calculation methods are available for the three aquatic trophic levels (fish, algae and aquatic invertebrates) to assess the toxicity of the test substance Reaction mass of dl-limonene, β-phellandrene.

Among the data available, a toxic effect was observed for these three aquatic trophic levels. The most sensitive species appears to be the aquatic invertebrateDaphnia magna, with a 48 h EC50 value estimated to be situated between 1.7 and 2.3 mg/L (loading) under experimental conditions, according to a reliable experimental study.

Moreover, based on calculation methods, the 96-h LL50 for Reaction mass of dl-limonene, β-phellandrene to the fishDanio reriois 4.1 mg/L and the 72 h ErL50 to the algaeP. subcapitatais 3.3 mg/L. For these calculations, the algorithms used were based on QSAR models which have been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004).

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