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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Key value determined using OECD no. 209, EU Method C11 and ISO Standard 8192

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, the test substance – Milli-RO water mixtures were magnetically stirred for a period of approximately 25 hours. Subsequently, synthetic medium, sludge and Milli-RO water were added resulting in the required loading rates. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period.


4-(1-methyl-1-phenylethyl)-N-[4-(1-methyl-1-phenylethyl)phenyl]aniline was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 1000 mg/l (NOEC).


The EC50 exceeded a loading rate of 1000 mg/l.