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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline Study with acceptable restrictions (non-GLP, unknown purity of TS).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
Semiocclusive and occlusive exposure of TS to rabbit skin for 3 min, 1 h and 4 h.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(dimethylamino)ethyl acrylate
EC Number:
219-460-0
EC Name:
2-(dimethylamino)ethyl acrylate
Cas Number:
2439-35-2
Molecular formula:
C7H13NO2
IUPAC Name:
2-(dimethylamino)ethyl acrylate
Details on test material:
- Name of test material (as cited in study report): Dimethylaminoethyl acrylate
- Purity: no data

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gaukler, Offenbach, FRG
- Weight at study initiation: mean males: 2.47 kg (3 min) and 3.06 kg (1 and 4 h); mean females: 2.57 kg (3 min) and 3.08 kg (1 and 4 h)
- Housing: single housing in wire mesh cages
- Diet: Solikanin 4 mm (MUSKATOR-WERKE, Düsseldorf, FRG), approx. 130 g per animal per day
- Water: approx. 250 ml per animal per day
- Acclimation period: approx. 8 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): 30 - 70
- Photoperiod (hrs dark / hrs light): 12 / 12

Test system

Type of coverage:
other: semiocclusive and occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent no treatment
Amount / concentration applied:
0.5 ml
Duration of treatment / exposure:
3 minutes, 1 hour and 4 hours
Observation period:
7 days
Number of animals:
6
Details on study design:
TEST SITE
- Area of exposure: 3 x 3 cm (dorsal/flank skin)


REMOVAL OF TEST SUBSTANCE
- Washing: Lutrol and Lutrol/water (1:1)
- Time after start of exposure: 3 min, 1 h, 4 h


SCORING SYSTEM: According to Draize

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
3.4
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Remarks on result:
other: 4 h semiocclusive exposure
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
3.4
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Remarks on result:
other: 4 h semiocclusive exposure

Any other information on results incl. tables

Irritation/corrosion readings after 4 h semiocclusive exposure:

Reading Erythema/Edema in animal No.

1

2

3

4

5

6

24 h

3/4

3/4

4/4

3/4

3/4

3/4

48 h

3/3

3/3

4/4

3/3

4/3

3/3

72 h

4/3

4/3

4/3

3/3

4/3

4/3

mean

3.3/3.3

3.3/3.3

4/3.7

3/3.3

3.7/3.3

3.3/3.3

3 minutes semiocclusive exposure lead to irritation, after 1 hour semiocclusive expoure necrosis were observed.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information
Conclusions:
Classification: corrosive (causes burns)