3-methyl-5-phenylpentanol

Repeated dose toxicity: other routes

Administrative data

Endpoint:

short-term repeated dose toxicity: other route

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

Not reported

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Performed using a non-toxicologically relevant route. Major areas of the reporting lack details i.e. purity of the substance not stated (supplier of the test material is reported and it is assumed that the substance would be a high grade); very limited details of the animals; dosages uncertain.

Data source

Reference

Reference Type:

publication

Title:

Regeneration of rat liver in the presence of essential oils and their components.

Author:

Gershbein LL

Year:

1977

Bibliographic source:

Food and Cosmetics Toxicology, 15 (3): 173–181.

Materials and methods

Principles of method if other than guideline:

The purpose of the study was to determine whether essential oils, flavourings, spices and plant materials could stimulate liver regeneration.
Partially hepatectomised rats (Charles River) were administered subcutaneously with 4-Methoxybenzyl alcohol (in peanut oil) daily for 7 days up to a total dose of 2450-2950 mg/kg. On the 10th day animals were sacrificed and the livers removed and dried to a constant weight at 100 °C. Samples of the removed livers were examined microscopically for changes.

GLP compliance:

not specified

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-methoxybenzyl alcohol

EC Number:

203-273-6

EC Name:

4-methoxybenzyl alcohol

Cas Number:

105-13-5

IUPAC Name:

(4-methoxyphenyl)methanol

Test material form:

not specified

Details on test material:

- Name of test material: 4-Methoxybenzyl alcohol

Test animals

Species:

rat

Strain:

other: Charles River

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Housing: Individually
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

subcutaneous

Vehicle:

peanut oil

Details on exposure:

Prior to treatment, the animals were partially hepatectomised under ether anaesthesia, removing two-thirds of the organ. The tissue was then dried to a constant weight at 100 °C.

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

7 days (rats were sacrificed after 10 days).

Frequency of treatment:

Daily

Doses / concentrations

Remarks:

Doses / Concentrations:
Up to a total dose of 2450-2950 mg/kg over the dosing period.

Control animals:

yes, concurrent vehicle

Examinations

Sacrifice and pathology:

On day 10, animals were sacrificed and the entire livers were removed and dried at 100 °C to a constant weight. The liver increment or the amount of tissue regenerated was calculated from the dry liver weight by taking the product of the weight of the surgery and a factor of 0.46 and subtracting this from the weight at autopsy. Small sections of the livers were retained for microscopic analysis.

Results and discussion

Results of examinations

Details on results:

Rats dosed with 4-methoxybenzyl alcohol did not demonstrate any stimulation of liver regeneration under the conditions of the study.

Target system / organ toxicity

Critical effects observed:

not specified

Applicant's summary and conclusion

Conclusions:

Rats dosed with 4-methoxybenzyl alcohol did not demonstrate any stimulation of liver regeneration under the conditions of the study.

Executive summary:

The purpose of the study was to determine whether essential oils, flavourings, spices and plant materials could stimulate liver regeneration.

Partially hepatectomised rats (Charles River) were administered subcutaneously with 4-Methoxybenzyl alcohol (in peanut oil) daily for 7 days up to a dose of 2450-2950 mg/kg/day. On the 10th day animals were sacrificed and the livers removed and dried to a constant weight at 100 °C. Samples of the removed livers were examined microscopically for changes. Rats dosed with 4-methoxybenzyl alcohol did not demonstrate any stimulation of liver regeneration under the conditions of the study.