Registration Dossier
Registration Dossier
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EC number: 429-270-1 | CAS number: 136210-30-5
Endpoint summary
Administrative data
Description of key information
Additional information
The acute toxicity of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards fish (Brachydanio rerio) was determined according to the OECD-Guideline 203 yielding a LC0(96 h) ≥ 5 mg/L as no adverse effects were observed testing concentrations up to the water solubility limit of the test item (Bayer AG, 1998b).
Aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester was tested with respect to the acute toxicity towards invertebrates (Daphnia magna) according to a national standard method: "Determination of the immobilization at daphnia", UBA, May 1984 yielding an EC50(48 h) ≥ 5 mg/L (Bayer AG, 1998c).
The chronic toxicity of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards reproduction of Daphnia magna was tested according to the EC-method C.20, No. 440/2008 yielding the following results (Currenta, 2009c):
21d-NOEC (reproduction): 0.013 mg/L 21d-LOEC (reproduction): 0.041 mg/L
21d-NOEC (mortality): >= 0.12 mg/L 21d-LOEC (mortality): > 0.12 mg/L
Aspartic acid, N,N’-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester was tested for toxicity towards algal growth according to the OECD-Guideline 201 yielding an ErC 50 (0 - 48 h) > 1.319 mg/L as no toxic effects were observed at testing concentrations up to the water solubility limit of the test item (Currenta, 2010b).
The effect of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards the growth of activated sludge was investigated according to ISO 8192 -1986 (E) having an EC50(3 h) of 3110 mg/L (Bayer AG, 1998d). Therefore, an adverse effect towards microorganisms and thus the cleaning capacity of sewage treatment plants is not expected.
All studies investigating the aquatic toxicity were performed with
aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transfered to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation.
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