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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not applicable
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
α,2-dichlorotoluene
EC Number:
210-258-8
EC Name:
α,2-dichlorotoluene
Cas Number:
611-19-8
Molecular formula:
C7H6Cl2
IUPAC Name:
1-chloro-2-(chloromethyl)benzene
Details on test material:
Source: Occidental Chemical Corporation

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 180 - 300 g
- Fasting period before study: approximately 16 to 22 hours prior to the treatment
- Housing: individually in wire mesh cages
- Diet: ad libitum
- Water: ad libitum
- Other conditions were set according to AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care) Standards

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12


Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Doses:
280, 390, 540, 790 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
Following administration, observations were made three times at the first day and daily thereafter for 14 days.
Any symptoms of toxicity of the animals were recorded.
Necropsy was made on all dead animals.
At 14 days all surviving animals are weighed, then they were sacrificed and necropsied.
Statistics:
LD50 with 95% confidence limits was calculated according to the method of C. S. Weil, Biometrics 249 (1952).

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
430
Based on:
test mat.
95% CL:
380 - 490
Mortality:
All male and female rats at 540 and 760 mg/kg bw, and three female at 390 mg/kg bw died at the first day.
Other rats were survived for 14 days.

Clinical signs:
Decreased motor activity and respiration, diarrhea, salivation and chromodacryorrhea
Body weight:
There were no effects on body weight.
Gross pathology:
There were no gross tissue changes observable at the necropsy.
Other findings:
The oral  LD50 with 95% confidence limits was calculated to be 430 (380 - 490) mg/kg bw.

Any other information on results incl. tables

Table 1. Mortality of rats treated orally with OCBC
===================================================
                         Number of deaths
            ---------------------------------------
Dose        Hours   Days
(mg/kg)     0-4     1     2    3     4-14    Total
            M/F     M/F   M/F  M/F   M/F      M/F
--------------------------------------------------------
280         0/0     0/0   0/0  0/0   0/0      0/0
390         0/0     0/3   0/0  0/0   0/0      0/3
540         0/0     5/5   -/-  -/-   -/-      5/5
760         0/0     5/5   -/-  -/-   -/-      5/5
===================================================

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information R22: Harmful if swallowed / GHS Cat. 4 Criteria used for interpretation of results: EU
Conclusions:
o-Chlorobenzylchloride is considered to be harmful following acute oral exposure (LD50 430 mg/kg bw (95% confidence limits: 380-490 mg/kg bw)).
Executive summary:

In an acute oral toxicity study o-Chlorobenzylchloride was given in a single dose by gavage to five rats per sex at doses of 280, 390, 540, and 760 mg/kg bw; animals were observed for 14 days. Decreased motor activity and respiration, diarrhea, salivation and chromodacryorrhea were noticed within the observation period. All male and female rats at 540 and 760 mg/kg bw, and three female at 390 mg/kg bw died at the first day. Surviving animals were sacrificed and necropsied after 14 days. There were influence on body weight development and no gross tissue changes observable at necropsy. The oral LD50 with 95% confidence limits was calculated to be 430 (380 - 490) mg/kg bw.