Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The mobility of aquatic invertebrates was not affected in a flow-through system at the highest concentration that could be maintained under test conditions with a mean measured concentrations of 5.4 mg/L after 48-hours.

 

Key value for chemical safety assessment

Additional information

The acute toxicity of the test substance 3,5,5-trimethylhexyl acetate to aquatic invertebrates (Daphnia magna) was investigated in two independent studies according to relevant OECD guidelines. The key study was conducted under flow-through conditions to maximise the test concentration of the volatile test item (Brougher et al. 2013). Test concentrations were measured and results were based on mean measured test concentrations, as the recovery of nominal concentrations was below 50% for all concentrations. No immobilisation of daphnids occurred at the highest concentration that could be maintained under test conditions with a mean measured concentration of 5.4 mg/L and the EC50 over the study duration of 48 hours was > 5.4 mg/L.

The supporting study exposed daphnids in a static system over a period of 48 hours (Caspers 2001), but the volatility of the test substance was not taken into account. The TOC was measured to confirm nominal test concentrations of 25, 50 and 100 mg/L. All measured concentrations were at or below the LOQ of the method (2 mg/L corresponding to 2.8 mg/L test substance) indicating deficiencies of the analytical method. At the highest nominal concentration of 100 mg/L, 30% of the daphnids were immobile after 48 hours. As the actual test concentration is not known, the results from this test (EC50 > 2.8 mg/L) are not taken into account in the hazard characterisation.