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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to guideline but without GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
Principles of method if other than guideline:
OECD 202 adopted 1984-04-04, part II
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
test concentration:
control 0.03 mg/l
0.1 mg/l
0.3 mg/l
1,0 mg/l
3.3 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia was sourced from Bundesgesundheitsamt Berlin, Klonzucht AWALU/Labor für Wassserbiologie
Breeding conditions: breeding was done in water bath under constant temperature (20 °C).
breeding water was conditioned tap water of Leverkusen
Feeding: with singular cell Scenedesmus subspicatus (out of fermenter breeding) ad libitum
before feeding ultrasound of 5 minutes, adapting of the algae suspension to extiguishing value of 0.54 at 578 nm in 1:10 solution
feeding of one adult per day: 0-1 day 0,10 ml feeding algae
2-3 day 0.12 ml feeding algae
4-5 day 0.16 ml feeding algae
6-7 day 0.20 ml feeding algae
8-20 day 0.40 ml feeding algae

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
no data

Test conditions

Test temperature:
20 °C
pH:
ph was meassured at test start and after each new set-up of the test medium
Dissolved oxygen:
O2 was meassured at test start and after each new set-up of the test medium

Results and discussion

Effect concentrations
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
R21 (control) 54,4 = 100 %
R21 (0,03 mg/l) 63.3 = 116,4 %
R 21 (0.1 mg/l) 44.3 = 81,4 %
R 21 (0.3 mg/l) 48,1 = 88.5 %
R 21 (1,0 mg/l) only Subitaneggs
R21 (3,3 mg/l) 100 % Mortaility

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
EC 50 for reproduction rate after 21 days was 4.0 mg/l.
Executive summary:

.

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