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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to guideline but without GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
Principles of method if other than guideline:
OECD 202 adopted 1984-04-04, part II
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
test concentration:
control 0.03 mg/l
0.1 mg/l
0.3 mg/l
1,0 mg/l
3.3 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia was sourced from Bundesgesundheitsamt Berlin, Klonzucht AWALU/Labor für Wassserbiologie
Breeding conditions: breeding was done in water bath under constant temperature (20 °C).
breeding water was conditioned tap water of Leverkusen
Feeding: with singular cell Scenedesmus subspicatus (out of fermenter breeding) ad libitum
before feeding ultrasound of 5 minutes, adapting of the algae suspension to extiguishing value of 0.54 at 578 nm in 1:10 solution
feeding of one adult per day: 0-1 day 0,10 ml feeding algae
2-3 day 0.12 ml feeding algae
4-5 day 0.16 ml feeding algae
6-7 day 0.20 ml feeding algae
8-20 day 0.40 ml feeding algae

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
no data

Test conditions

Test temperature:
20 °C
pH:
ph was meassured at test start and after each new set-up of the test medium
Dissolved oxygen:
O2 was meassured at test start and after each new set-up of the test medium

Results and discussion

Effect concentrations
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
R21 (control) 54,4 = 100 %
R21 (0,03 mg/l) 63.3 = 116,4 %
R 21 (0.1 mg/l) 44.3 = 81,4 %
R 21 (0.3 mg/l) 48,1 = 88.5 %
R 21 (1,0 mg/l) only Subitaneggs
R21 (3,3 mg/l) 100 % Mortaility

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
EC 50 for reproduction rate after 21 days was 4.0 mg/l.
Executive summary:

.