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EC number: 700-991-6 | CAS number: 8007-24-7
Endpoint summary
Administrative data
Description of key information
Phototransformation in air
The atmospheric oxidation of test chemical half-lifewas estimated using the level III multimedia model. It was estimated that test chemical shows rapid degradation in the air medium as the half life period of test chemicalin air was reported as 0.9275 hours.
Hydrolysis
The hydrolysis rate constant of test chemical was estimated to be 0.000000000155 cm3/molecule-sec in half life of 0.826 Hrs .
Phototransformation in water
No experimental data available. As this study is not a standard information requirement in REACH and there is no indication from the CSA on the need to investigate further the fate and behaviour of the substance (Annex X requirement), no further testing is considered necessary.
Phototransformation in soil
No experimental data available. As this study is not a standard information requirement in REACH and there is no indication from the CSA on the need to investigate further the fate and behaviour of the substance (Annex X requirement), no further testing is considered necessary.
Biodegradation in water:
28-days Manometric respirometry test was performed by following the OECD guideline 301F to determine the ready biodegradability of the test chemical. The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 50 mg/L and 100 mg/L respectively, while that of inoculum was 30.ml/L. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Acetate) was also calculated using BOD & ThOD and was determined to be 96.80 %. The DO depletion (mg O2/l) in control on 28th day was 19.1 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium acetate exceeds 67 %after 7 days and 78 % after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.73 mgO2sup>/mg. ThOD was calculated as 3.067 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 23.80 %. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20 ± 1°C over a period of 28 days.
Biodegradation in Water & Sediment:
Estimation Programs Interface (2018) prediction model was run to predict the half-life in water and sediment for the test chemical . If released in to the environment, 7.79 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is low /whereas the half-life period of test chemical in sediment is estimated to be 337.5 days ( 8100 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in Soil:
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (2018). If released into the environment, 48.2 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low .
Bioaccumulation: aquatic / sediment
BCFBAF Program of Estimation Programs Interface was used to predict the bioconcentration factor (BCF) of test chemical. The bioconcentration factor (BCF) of test chemical was estimated to be 483.6 L/kg whole body w.w (at 25 deg C),which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.
Adsorption/Desorption:
The soil adsorption coefficient i.e Koc value of test chemical was estimated to be 122.513 (log Koc=2.088). This Koc value indicates that the test chemical has a low sorption to soil and sediment and therefore have moderate migration potential to ground water
Henry's Law Constant
The estimated Henry's Law Constant value of the test chemical is 0.000499301 Pa-m3/mole.
Additional information
Phototransformation in air
The atmospheric oxidation of test chemical half-lifewas estimated using the level III multimedia model. It was estimated that test chemical shows rapid degradation in the air medium as the half life period of test chemicalin air was reported as 0.9275 hours.
Hydrolysis
The hydrolysis rate constant of test chemical was estimated to be 0.000000000155 cm3/molecule-sec in half life of 0.826 Hrs .
Phototransformation in water
No experimental data available. As this study is not a standard information requirement in REACH and there is no indication from the CSA on the need to investigate further the fate and behaviour of the substance (Annex X requirement), no further testing is considered necessary.
Phototransformation in soil
No experimental data available. As this study is not a standard information requirement in REACH and there is no indication from the CSA on the need to investigate further the fate and behaviour of the substance (Annex X requirement), no further testing is considered necessary.
Biodegradation
Biodegradation in Water:
28-days Manometric respirometry test was performed by following the OECD guideline 301F to determine the ready biodegradability of the test chemical. The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 50 mg/L and 100 mg/L respectively, while that of inoculum was 30.ml/L. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Acetate) was also calculated using BOD & ThOD and was determined to be 96.80 %. The DO depletion (mg O2/l) in control on 28th day was 19.1 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium acetate exceeds 67 %after 7 days and 78 % after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.73 mgO2sup>/mg. ThOD was calculated as 3.067 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 23.80 %. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20 ± 1°C over a period of 28 days.
Biodegradation in Water & Sediment:
Estimation Programs Interface (2018) prediction model was run to predict the half-life in water and sediment for the test chemical . If released in to the environment, 7.79 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is low /whereas the half-life period of test chemical in sediment is estimated to be 337.5 days ( 8100 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in Soil:
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (2018). If released into the environment, 48.2 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low .
Bioaccumulation: aquatic / sediment
Predicted data and experimental study of the test chemical were reviewed for the bioaccumulation end point which are summarized as below:
In a prediction done using the BCFBAF Program of Estimation Programs Interface was used to predict the bioconcentration factor (BCF) of test chemical. The bioconcentration factor (BCF) of test chemical was estimated to be 483.6 L/kg whole body w.w (at 25 deg C),which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.
For the test chemical, the bioaccumulation study was conducted on test organism Cyprinus carpio for 60 days for evaluating the bioconcentration factor (BCF value) oftest chemical.Cyprinus carpio was used as a test organism for the study. Range finding study involve the LC50(96 h) 0.198 mg/lonRice fish (Oryzias latipes). The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be 460 L/Kg at a conc. of 0.001 mg/l, respectively.
On the basis of above results for test chemical, it can be concluded that the BCF value of test chemical was evaluated to beupto 460,which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.
Adsorption/Desorption:
The soil adsorption coefficient i.e Koc value of test chemical was estimated to be 122.513 (log Koc=2.088). This Koc value indicates that the test chemical has a low sorption to soil and sediment and therefore have moderate migration potential to ground water
Henry's Law Constant
The estimated Henry's Law Constant value of the test chemical is 0.000499301 Pa-m3/mole.
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