Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010-03-3 / 2010-04-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to the OECD Guideline and it is GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Amines, C20-22-alkyldimethyl
EC Number:
296-866-4
EC Name:
Amines, C20-22-alkyldimethyl
Cas Number:
93164-85-3
Molecular formula:
CnH2nN(CH3)2 n: from 18 to 22
IUPAC Name:
Amines, C20-22-alkyldimethyl
Details on test material:
- Physical state: white solid
- Storage: at room temperature in the dark under nitrogen
- Stability: stable

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: activated sludge freshly, sewage treatment plant
Details on inoculum:
The substance was tested in duplicate at 16 mg/L. corresponding to 13 mg TOC/L.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
13 mg/L
Based on:
other: TOC

Results and discussion

% Degradationopen allclose all
Value:
7
Sampling time:
28 d
Value:
12
Sampling time:
28 d

Any other information on results incl. tables

 Test medium  At the start of the test  On day 28
 Blank control (A)  7.7 - *7.6  8.0
 Blank control (B)  7.8 - *7.6  7.9
 Positive control  7.7- *7.6  8.1
 The substance (A)  7.7- *7.6  7.9
 The substance (B)  7.8- *7.6  7.9
 Toxicity control  7.7- *7.6  8.1

* pH adjuested with 1 M HCl

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
The substance was not readily biodegradable under the conditions of the modified Sturm test presently performed.
Executive summary:

The study procedures describes in this report were based on the OECD Guideline 301B. The study consisted of six bottles, 2 inoculum, 2 test bottles, 1 positive control and 1 toxicity control.Since the substance was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2 -litres test bottles containing medium with microbial organisms and mineral components. The relative biodegradation values calculated from the measurements performed during the test period revealed 7 and 12% biodegradation of the substance, for the duplicate bottles tested. The substance is designated as not readily biodegradable.