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EC number: 296-866-4 | CAS number: 93164-85-3
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 23 March 2010 to 15 April 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to the OECD Guideline and it is GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Amines, C20-22-alkyldimethyl
- EC Number:
- 296-866-4
- EC Name:
- Amines, C20-22-alkyldimethyl
- Cas Number:
- 93164-85-3
- Molecular formula:
- CnH2nN(CH3)2 n: from 18 to 22
- IUPAC Name:
- Amines, C20-22-alkyldimethyl
- Details on test material:
- -Physical state: clear colourless liquid
- Substance storage: at room temperature in the dark under nitrogen
- Stabilty under storage conditions: stable
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera)
- Source: In-house laboratory culture with a known history
- Maximum age of the cultures: 4 weeks
- Temperature of medium: 18-22ºC
- Feeding: Daily, a suspension of fresh water algae
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- pH:
- 7.7
- Dissolved oxygen:
- 9.3
- Nominal and measured concentrations:
- 4.6, 10, 22, 46 and 100% of a WSF prepared at a loading rate of 100 mg/L.
control: test medium without test substance or other additives - Details on test conditions:
- - Test vessels: 100mL, all-glass
- Number of daphnids: 20 per concentration
- Loading: 5 per vessel containing 80 mL of test solution
- Light: 16 hours photoperiod daily
- Feeding: not feeding
- Aeration: No aeration of the test solutions - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.46 mg/L
- Conc. based on:
- test mat.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.21 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.092 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Acute immobilisation of daphnids after 24 and 48 hours in the final test
| %WSF / Actual (mg/L) | Vessel number | Number Daphnia exposed | Response | at 24 h | Response | at 48 h |
| number | total % | number | total response | |||
| control | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| C | 5 | 0 | 0 | |||
| D | 5 | 0 | 0 | |||
| 0.1 / 0.003 | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| 1.0 / 0.03 | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| 10 / 0.32 | A | 5 | 0 | 0 | 2 | 20 |
| B | 5 | 0 | 0 | |||
| 100 / 3.2 | A | 5 | 3 | 55 | 5 | 100 |
| B | 5 | 3 | 5 | |||
| C | 5 | 2 | 5 | |||
| D | 5 | 3 | 5 |
Acute immobilisation of daphnids after 24 and 48 hours in the final test
| %WSF / Actual (mg/L) | Vessel number | Number Daphnia exposed | Response | at 24 h | Response | at 48 h |
| number | total % | number | total response | |||
| control | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| C | 5 | 0 | 0 | |||
| D | 5 | 0 | 0 | |||
| 4.6 / n.a | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| C | 5 | 0 | 0 | |||
| D | 5 | 0 | 0 | |||
| 22 / 0.03 | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| C | 5 | 0 | 0 | |||
| D | 5 | 0 | 0 | |||
| 46 / 0.08 | A | 5 | 0 | 0 | 0 | 0 |
| B | 5 | 0 | 0 | |||
| C | 5 | 0 | 2 | |||
| D | 5 | 0 | 1 | |||
| 100 / 0.21 | A | 5 | 0 (1) | 0 | 5 | 100 |
| B | 5 | 0 | 5 | |||
| C | 5 | 0 | 5 | |||
| D* | 5 | 0 | 6 |
n.a not analysed
n.d not determined
* Inadvertently 6 organisms to this vessel
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study the substance induce ≤ 10% acute immobilisation of Daphnia magna at 0.03 mg/L after 48 hours of exposure. The 48h-EC50 was estimated to correspond to 0.092 mg/L based on average exposure concentrations.
- Executive summary:
The study procedures described in this report were based on the OECD guideline 202 and the test methods of the Commission Regulation (EC) No 440/2008, Part C.2, 2008.Twenty daphnia per test group (5 per vessel, 4 vessels) were exposed to a control and a dilution series representing 4.6, 10, 22, 46 and 100% of a WSF prepared at a loading rate of 100 mg/L.Measured concentrations were 0.016, 0.046, 0.12 and 0.28 mg/L in the groups containing 10, 22, 46 and 100% of the WSF, respectively. The concentrations measured after 48 hours decreased by approximately 50% in the three highest groups, while the measured concnetration for the 10% WSF decreased below the LOD (<0.00017 mg/L).
The 48 -h EC50 was estimated to correspond to 0.092 mg/L based on average exposure concentrations.
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