Octane-1,2-diol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-compliant OECD guideline study; short report on results from a screening test (range finding study); deemed scientifically acceptable based on results.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

, version of 17.07.1992

Deviations:

not applicable

Remarks:

(range-finder was performed only)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Before use, the test material was heated in a water bath at about 30-40°C. About 8.8 g were weighed and dissolved in 4 L of dilution water to receive a stock solution of 2.2 g/L.
- Controls: dilution water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain: CRL / ZF1
- Source: Bio-International, The Netherlands
- Length at study initiation (length definition, mean, range and SD): 2.0±1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period: 30 days
- Type and amount of food: TetraMin fish food; no feeding during the 24 hours before exposure
- Health during acclimation (any mortality observed): no mortality observed

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not indicated

Test temperature:

22±2 °C (air-conditioned laboratory

pH:

7.5-8.0

Dissolved oxygen:

7.2-8.5 mg/L (>60% of saturation concentration)

Salinity:

not applicable

Nominal and measured concentrations:

nominal: 0.0022, 0.022, 0.22 and 2.2 g/L (2.2, 22, 220 and 2200 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: not indicated
- Material, size, headspace, fill volume: 2 L
- Aeration: no
- No. of organisms per vessel: 3 (range-finder test)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water
- Culture medium different from test medium: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and sub-lethal effects; recorded at 0, 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: based on the results obtained during the range finding study the main study was not conducted (range finding study results are reported)
- Range finding study
- Test concentrations: undiluted stock solution (nominal 2.2 g/L) and dilutions 1:10 (nominal 220 mg/L), 1:100 (nominal 22 mg/L) and 1:1000 (nominal 2.2 mg/L);
- Results used to determine the conditions for the definitive study: the definitive study was not conducted (see Executive summary)

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2.2 - < 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

< 2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1 out of 3 fish died

Details on results:

- Behavioural abnormalities: none
- Mortality of control: not reported
- Abnormal responses: none
- Effect concentrations exceeding solubility of substance in test medium: the stock solution (used as the highest concentration in this range-finding study) showed a hardly observable oily phase at the surface and was slightly turbid.

Reported statistics and error estimates:

not applicable

Sublethal observations / clinical signs:

Table 1 Mortality

 

Dilution	Nominal	concentration	Number of dead	Remarks
	g/L	mg/L	fish after 96 h
undiluted	2.2	2200	3	the first fish died after ca. 10 min.
1:10	0.22	220	3	fish were dead after ca. 25 min. already
1:100	0.022	22	3	two fish were dead after 72 h
1:1000	0.0022	2.2	1	--

 

Initial population = 3 fish / concentration. First, one of the three fish was inserted into each vessel. After about 10 minutes the remaining fish were inserted if no damage was observed.

Validity criteria fulfilled:

not applicable

Executive summary:

The reported data are from a range finding study. Since a LC50 in the range of 2 mg/L was expected, the originally foreseen analytical method (TOC analysis) was regarded as not sensitive enough. In the course of the development of a new analytical method it appeared that the test material was not stable in the relevant concentration range. Thus, the study was cancelled since a completely new test-design (flow-through system) would have been needed.

The results obtained in this range study are sufficiently meaningful. The expected LC50 is above 1 mg/L and thus would not trigger classification of the substance since the test material is readily biodegradable with a Log Pow of 2.1.

In the light of animal welfare a new full acute toxicity study with fish is not needed.

Description of key information

96-h LC50 2.2-22 mg/L for Brachydanio rerio (OECD TG 203)