Ethylene dinitrate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Proposed ASTM method for 3-brood renewal toxicity test (Draft 3).

Deviations:

not specified

GLP compliance:

no

Remarks:

Study is based on a standard national protocol with no variations or quality control issues and is very well-documented. Note that it is unlikely that the laboratory was operating according to all GLP principles at the time of the testing.

Test material

Test material information

Constituent 1

Reference substance name:

Glycerol trinitrate

EC Number:

200-240-8

EC Name:

Glycerol trinitrate

Cas Number:

55-63-0

Molecular formula:

C3H5N3O9

IUPAC Name:

propane-1,2,3-triyl trinitrate

Specific details on test material used for the study:

NG dissolved in JHU/APL well water at 1g/L was obtained from the US Naval Ordnance Section, Indian Head, MD.
Reverse phase HPLC determined that no impurities were present during testing.
Stability of stock solution was checked periodically during the study period.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

Test species was Ceriodaphnia dubia cultured at 25 °C and fed a diet of Cerophyl and green algae. Starter cultures were obtained from Center for Lake Superior Environmental Studies, University of Wisconsin.
1 daphnid neonate was randomly assigned to each control or treatment replicate.
Test organisms were fed diet described above before the test, at test initiation and at each 24 h renewal time.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

7 d

Test conditions

Details on test conditions:

Acute test with static renewal system with renewal at 24 hrs.
The negative control with 10 replicates was run concurrently.

Photoperiod, temperature, DO, pH, alkalinity, conductivity and total hardness were monitored and reported.
Photoperiod was 16:8 light:dark.
Mean and range of temperature and water quality parameters fell within ranges specified in study guideline.

10 replicates were used per treatment.
Exposure period was 7 days
Exposure pathway was aqueous
Geometric dilution series of 5 concentrations was tested.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

3.23 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

7 d

Dose descriptor:

LOEC

Effect conc.:

5.48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Endpoint = 7-d LOEC = 5.48 mg/L; 7-d NOEC = 3.23 mg/L
Effect = reproduction
Significant mortality of adults observed in treatment with 16.05 mg/L NG

Reported statistics and error estimates:

Statistical analysis of concentration data and organism response was based on mean measured concentrations of NG.
Neonate production data were not normally distributed (Chi-square test for normality p<0.05) and therefore, Steel’s Many-One Rank Test (non-parametric) test was conducted to determine which dilutions were significantly different from the control (alpha = 0.05).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes