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EC number: 249-951-5 | CAS number: 29911-28-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April-December 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline 475
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Principles of method if other than guideline:
- Method: other: No specific protocol guideline cited (e.g., OECD 475: "Mammalian Erythrocyte Micronucleus Test"). General guidelines cited: 40 CFR Part 160, OECD ISBN 92-64-12367-9.
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1-(2-butoxy-1-methylethoxy)propan-2-ol
- EC Number:
- 249-951-5
- EC Name:
- 1-(2-butoxy-1-methylethoxy)propan-2-ol
- Cas Number:
- 29911-28-2
- Molecular formula:
- C10H22O3
- IUPAC Name:
- 1-(2-butoxy-1-methylethoxy)propan-2-ol
- Details on test material:
-
Identity: Dowanol-DPnB (n-butoxypropoxypropanol or
dipropylene glycol normal-butyl ether).
CAS # 29911-28-2
Appearance: Liquid.
Batch No.: QA001078 (produced in July 1987).
Source: Dow Chemical Europe, Stade, Federal Republic of Germany.
Expiration Date: Not specified
Purity: 99.5%
Specific Gravity: Not specified
Solubility in
water: Not specified
Stability: Not specified
Storage: Not specified
Administered as: Dilution in 1% Methocel and water.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: CD-1 (ICR) BR
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- 1% Methocel*
- Duration of treatment / exposure:
- Single administration
- Frequency of treatment:
- Single administration
- Post exposure period:
- Animals were sacrificed at 24 h, 48 h, 72 h post exposure to test material.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 250, 833, 2500 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 5 animals per sex per dose per sacrifice time
- Control animals:
- yes
- Positive control(s):
- 120 mg/kg bw cyclophosphamide
Examinations
- Tissues and cell types examined:
- Bone marrow was collected from the femur of each animal. Cells from the bone marrow were transferred to slides, fixed in methanol, and stained in 5% Giemsa. One thousand polychromatic erythrocytes were evaluated from each animal and the frequencies of micronucleated polychromatic erythrocytes were recorded.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not examined
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- There were no significant increases in the frequencies of micronucleated polychromatic erythrocytes (MN-PCE) in any of the groups treated with the test chemical compared to negative controls at any dose or time point. The positive control mice showed significant increases in MN-PCE.
Other metabolism studies show that DPnB reaches the bone marrow of mice. This in vivo assay confirms that that DPnB is not clastogenic to chromosomal material. See also General Remark above in the In Vitro Genotoxicity section.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
Under the experimental conditions used, the test chemical was negative in the mouse bone marrow micronucleus test. - Executive summary:
Groups of CD-1 (ICR) BR mice (5/sex/dose/sacrifice time) were administered single doses (by gavage) of 0, 250, 833,
or 2500 mg DPnB/kg body weight. The positive control chemical was cyclophosphamide (120 mg/kg). The negative
control chemical (i.e., the diluent for DPnB) was 1% methocel (10 ml/kg). Groups of animals were sacrificed by
cervical dislocation at three time intervals: 24, 48, and 72 hours after treatment. Bone marrow was collected from the
femur of each animal. Cells from the bone marrow were transferred to slides, fixed in methanol, and stained in 5%
Giemsa. One thousand polychromatic erythrocytes were evaluated from each animal and the frequencies of
micronucleated polychromatic erythrocytes were recorded.There were no significant increases in the frequencies of micronucleated polychromatic erythrocytes (MN-PCE) in any of
the groups treated with the test chemical compared to negative controls at any dose or time point. The positive
control mice showed significant increases in MN-PCE.In conclusion, under the experimental conditions used, the test chemical was negative in the mouse bone marrow micronucleus test.
Other metabolism studies show that DPnB reaches the bone marrow of mice. This in vivo assay confirms that that DPnB
is not clastogenic to chromosomal material.
This study was identified as key for this toxicity endpoint because of the methods followed (which were well documented
in the report). The report included GLP and Quality Assurance statements, signed by the Study Director and Head
of the QA Unit, respectively. The cell line used, test substance concentrations and dose spacing (4 dose levels
including negative control, with highest being 2500 mg/kg), positive control agent used, the number of cells scored, and
scoring criteria all followed or exceeded guidance as specified in OECD Guideline 475 "Mammalian Erythrocyte
Micronucleus Test." The positive control agents gave the expected results showing that the animals were responsive to
clastogenic insult.
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