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Acute data, based on international guidelines, are available for the three aquatic trophic levels: fish, invertebrates and algae.

To rainbow trout Oncorhynchus mykiss, under static condition, the 96h-LC50 value was determined at 24 mg/L. To aquatic invertebrates Daphnia magna, under static condition, the 48h-EC50 value was determined at 53 mg/L. To the freshwater green algae species Pseudokirchneriella subcapitata, under static condition, the 72h-NOEC and 72h-ErC50 values based on growth rate were 7.6 mg/L and 61 mg/L, respectively.

Among all the species tested, a toxic effect was found at the three trophic levels, and the most sensitive species was the rainbow trout.

In addition, the toxicity of TBEP to microorganisms (activated sludge) was evaluated under static condition. the 3h-EC50 value was found to be higher than 1000 mg/L and the 3h-EC10 value was calculated to be 89.6 mg/L.