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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Secondary source

Data source

Reference
Reference Type:
secondary source
Title:
BUA, Beratergremium für umweltrelevante Altstoffe; 3,3´-Dichlrorobenzidin, BUA-Stoffbericht 30, March 1989
Year:
1989
Bibliographic source:
Gesellschaft Deutscher Chemiker, GdCh, Beratergremium für umweltrelevante Altstoffe, BUA, VCH Verlag, Weinheim

Materials and methods

Test material

Constituent 1
Reference substance name:
3,3'-dichlorobenzidine
EC Number:
202-109-0
EC Name:
3,3'-dichlorobenzidine
Cas Number:
91-94-1
IUPAC Name:
3,3'-dichlorobiphenyl-4,4'-diamine

Results and discussion

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Executive summary:

Toxicity of 3,3´-dichlorobenzidine had been tested to different micororganisms at pH 6.7, each:

  • Spirillum volutans, inhibition of mobility after 2 hours incubation: EC90 = 16 mg/L;
  • Pseudomonas fluorescens, growth inhibition after 18 hours incubation: EC50 >= 100 mg/L;
  • Aeromonas hydrophila, growth inhibition after 18 hours incubation: EC50 >100 mg/L;
  • Artificial activated sludge derived from 6 different bacterial species, isolated before from domestic and industrial sewage: EC50 > 100 mg/L;

    In a further test with Photobacterium phosphoreum, the effect of 3,3´-dichlorobenzidine on light emission was tested: EC50 = 0,058 mg/L.

    Aerobic bacteria from industrial activated sludge showed no effect at nominal concentrations of 3,3´-dichlorobenzidine between 20 -30 mg/L (Sapromat-test).

    For all tested concentrations above the solubility in water of 3,3´-dichlorobenzidine (3 mg/L) solutions were oversaturated or precipitate had been present