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Three studies are available on the toxicty of heptane to terrestrial plants. However, only the study by Hulzebos et al. (1993) is considered reliable. Lettuce (Lactuca sativa) seeds were exposed to soil treated with heptane at concentrations of 1000 mg/kg (soil dry weight). Examination of germination on day 7 and 14 showed no effects. The authors also assessed germination in a nutrient solution containing heptane. On day 21 the LC50 results for heptane were: 1.7 mg/L (test 1) and 47 mg/L (test 2).


For the purpose of risk assessment, soil PNECs for hydrocarbon blocks incorporated into the PETRORISK model have been derived using aquatic PNECs and the equilibrium partitioning method (EqP). The outcome of the risk assessment with RCRs far below 1, i.e. regional RCRs of 5.7E-08 and 5.2E-10 for agricultural soil and grassland, respectively, and local RCRs for agricultural soil in the range of 3.5E-05 and 5.8E-08 do not indicate a risk to the terrestrial environment (see localCSR and regionalCSR in PETRORISK spreadsheet attached in IUCLID section 13). Thus, no further testing is required.