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EC number: 202-267-0 | CAS number: 93-68-5
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Based on the nominal concentrations of the test item 2'-methylacetoacetanilide in the key study, the 48-h EC50 for Daphnia magna was estimated to be 931 mg/L.
The NOEC after 48 h was determined to be 667 mg/L as the highest tested concentration with no biologically effect.
In the supporting study the 48-h LC50 for Daphnia magna was estimated to be 278.3 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 931 mg/L
Additional information
Both studies were published in the OECD SIDS dossier in year 2003. The key study was conducted and reported according to the OECD Guideline for the testing of chemicals 202 and principles of GLP in year 1999.
In the acute immobilization test withDaphnia
magna(Crustacea) there are no effects of the test item.
The study was conducted under static conditions over a period of 48 h with 5 nominal test item concentrations ranging from 198 to 1000 mg/L
The test organisms were exposed to
each test concentration and control.
All effect values are based on the nominal concentrationsof
the test item 2'-methylacetoacetanilide. The water
quality parameters (pH-value and dissolved oxygen concentration),
measured at 0 and 48 h, were determined to be within the acceptable
limits. The validity criteria of the test guideline were fulfilled.
The supporting study was conducted and reported in year 1975, no Test Guideline is available.
In the acute immobilization test withDaphnia magna(Crustacea) there are effects after 96 hr exposure of the test item.
The study was conducted under static conditions over a period of 96 h with 3 nominal test item concentrations ranging from 10 to 1000 mg/L
The test organisms were exposed to
each test concentration and control (0 mg/L).
All effect values are based on the nominal concentrations of
the test item 2'-methylacetoacetanilide.
Immobility of the control was 20% (4 in 20) at 96 hr, and the partially low dissolved oxygen concentration may have contributed to the toxicity.
Furthermore, the actual concentrations of this test substance have not confirmed during this study.
The quality of this study is questionable and thereforevalidity criteria of the test guideline were not fulfilled.
Thus, the result of the key study will used for evaluation.
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