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Ecotoxicological information

Toxicity to microorganisms

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toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December 26, 1997
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
other: OECD Guideline 301 C(Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L
Test type:
other: Readily biodegradability
Water media type:
Limit test:
Total exposure duration:
28 d
Details on test conditions:
Test equipment: Improved type for a volatile substances

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): BOD and test item concentration by GC.

- Test concentrations: 100 mg/L.
Reference substance (positive control):
not specified
Key result
28 d
Dose descriptor:
Effect conc.:
100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
other: % biodegradability

Test item 2 -methylcyclohexyl acetate was readily biodegradable under test conditions.

BOD (28 days): 99% degradation

Test material analysis by GC (28 days): 100% degradation.

Validity criteria fulfilled:
not specified
The NOEC for microorganism toxicity of the test item 2-methylcyclohexyl acetate was determined to be 100 mg/L.
Executive summary:

A readily biodegradation test was performed on 2 -methylcyclohexyl acetate by MITI according to OECD Guideline 301 C. 30 mg/L of activated sludge were exposed to 100 mg/L test item for 4 weeks. The biodegradation obtained by indirect analysis was 99% (basis for effect: BOD), and by direct analysis 100% (basis for effect: GC) and therefore, 2 -methylcyclohexyl acetate was determined to be readily biodegradable. According to the current guidelines, the NOEC for microorganism toxicity was determined to be 100 mg/L since the compound degrades well in the ready biodegradability study.

Description of key information

Key study: Test method OECD 301C. No data on GLP. The NOEC (28 days) for 2-methylcyclohexyl acetate was determined to be 100 mg/L (basis for effect: biodegradation).

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

Key study: The NOEC (28 days) for 2 -methylcyclohexyl acetate was determined to be 100 mg/L since the compound degrades well (99% biodegradation by BOD analysis, 100% biodegradation by GC analysis) in a ready biodegradability test according to OECD 301 C.