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Diss Factsheets

Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Catalysis by cytochrome P-450 of an oxidative reaction in xenobiotic aldehyde metabolism: deformylation with olefin formation
Author:
Roberts E.S.
Year:
1991
Bibliographic source:
Proc Natl Acad Sci U S A;88(20):8963-6.

Materials and methods

Objective of study:
metabolism
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Isobutyraldehyde
EC Number:
201-149-6
EC Name:
Isobutyraldehyde
Cas Number:
78-84-2
Molecular formula:
C4H8O
IUPAC Name:
2-methylpropanal
Details on test material:
- Name of test material: Isobutyraldehyde
Radiolabelling:
no

Administration / exposure

Details on study design:
The individual P-450 cytochromes were purified from either rabbit nasal microsomes or liver microsomes. Isobutyraldehyde was incubated with the P-450 enzymed for 20 minutes at 37°C. All hydrocarbon products were analyzed by GC by comparison to authentic standards.

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
Isobutyraldehyde was converted to propylene by cytochrome P-450

Applicant's summary and conclusion