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Administrative data

Description of key information

One key study (Hine, 1970; Klimisch score=2) was identified to support skin and eye irritation endpoints.

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Additional information

In a key skin irritation study (Hine, 1970; Klimisch score=2), commercial hexane solvent containing between 5 and 80% n-hexane was applied to both intact and abraded skin of 6 male rabbits. The test area was covered with gauze for 24 hrs. After the exposure period, the animals were scored for irritation at 24 and 72 hrs after start of exposure. The primary dermal irritation index (PDII) was 1.92 which is calculated from the average erythema score and the average oedema score.

In a key eye irritation study (Hine, 1970; Klimisch score=2), approximately 0.1 ml of commercial hexane containing between 5 and 80% n-hexane was placed in the eyes of six male rabbits. The rabbits were examined for signs of irritation at 24, 48, and 72 hrs after start of exposure. Only 1 animal showed signs of irritation (score of 1) at 24 hrs. The mean conjunctivae, chemosis, iris, and cornea scores were 0.33, 0.0, 0.0, and 0.0 respectively.  This substance is therefore classified as not irritating under EU guidelines.

Justification for classification or non-classification

Based on evaluation of all the irritation toxicity data discussed above,Hydrocarbons, C5-C7, n-alkanes, iso-alkanes, cyclic, n-hexane rich, isclassified as a Category 2 skin irritant, H315: Causes skin irritation underCLP EU Regulation 1272/2008 (GHS aligned) based on the mean values of the skin irritant effects of oedema and erythema in rabbits.

Although data does not indicate that hydrocarbons, C5-C7, n-alkanes, iso-alkanes, cyclic, n-hexane rich, is irritating to the skin, it is classified as Xi; R38 Irritant; Irritating to skin as defined by Annex 1 of EU Dangerous Substances Directive 67/548/EEC and so classification has been applied.