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EC number: 234-123-8 | CAS number: 10543-57-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-11-06
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to OECD guideline 209. It followed the principles of GLP, but some parts of the study were not fully conducted under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- but GLP conform
Test material
- Reference substance name:
- N,N'-ethylenebis[N-acetylacetamide]
- EC Number:
- 234-123-8
- EC Name:
- N,N'-ethylenebis[N-acetylacetamide]
- Cas Number:
- 10543-57-4
- Molecular formula:
- C10H16N2O4
- IUPAC Name:
- N,N'-ethylenebis[N-acetylacetamide]
- Details on test material:
- - Name of test material (as cited in study report): T A E D
- Analytical purity: 99 +/- 0.5 %
- Purity test date: 24.08.1992
- Lot/batch No.: 24081
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- No test solution was prepared. The test material was given directly to the test vessels.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Origin: obtained from a local sewage processing plant (handles with predominantly domestic sewage) of Frankfurt-Sprendlingen, Germany
Maintenance before test start: according to corresponding Guideline
Acclimatization: not acclimatized to test item or reference substance
Study design
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 +- 2°C
- pH:
- 7.2 +- 0.2
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- 500 mg TAED/L to 1000 mg TAED/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- normal laboratory equipment,
- Aeration: yes, 0.5 to 1 litre per minute and test vessel.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1600 mg/L dry weight of microbial innoculum
EFFECT PARAMETERS MEASURED: respiration rate after 3 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: < 3.2
- test item concentrations were between 500 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Results with reference substance (positive control):
- The respiration rates of the controls were within the accepted range.
- Reported statistics and error estimates:
- no data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- TAED, at concentrations up to 1000 mg/L, exhibited no acute toxic effects towards the microbiological inoculum.
- Executive summary:
This study was designed to determine the effects o f TAED on sewage sludge micro-organisms by measuring the respiration rate after exposure to the test substance at concentrations of 500 to 1000 mg/L after a 3 h contact time.
TAED, at concentrations up to 1000 mg/L, exhibited no acute toxic effects towards the microbiological inoculum.
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