Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrithione zinc
EC Number:
236-671-3
EC Name:
Pyrithione zinc
Cas Number:
13463-41-7
Molecular formula:
C10H8N2O2S2Zn
IUPAC Name:
Bis [1-hydroxy-2(1H)-pyridinethionato-O,S](T-4)-zinc
Details on test material:
Purity: 97.9%

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
also T102
Metabolic activation:
with
Metabolic activation system:
S9
Test concentrations with justification for top dose:
Experiment 1, strains TA 1535, TA 1537, TA 98, TA 100:
0; 6.25; 12.5; 25.0; 50.0; 100 µg/plate
Experiment 1, strain TA 102:
0; 3.13; 6.25; 12.5; 25.0; 50 µg/plate
Experiment 2, strains TA 1535, TA 1537, TA 98, TA 100:
0; 1.56; 3.13; 6.25; 12.5; 25.0; 50.0 µg/plate
Experiment 2, strain TA 102:
0; 1.56; 3.13; 6.25; 12.5; 25.0; 35.0 µg a.s./plate
Vehicle / solvent:
dimethylsulphoxide
Controlsopen allclose all
Positive controls:
yes
Positive control substance:
sodium azide
Positive controls:
yes
Positive control substance:
9-aminoacridine
Positive controls:
yes
Positive control substance:
2-nitrofluorene
Positive controls:
yes
Positive control substance:
other: 2-aminoanthracene
Positive controls:
yes
Positive control substance:
cumene hydroperoxide
Positive controls:
yes
Positive control substance:
other: dimethylsulphoxide
Details on test system and experimental conditions:
experiment 1 = plate-incorporation method
experiment 2 = pre-incubation method (30 min)
Evaluation criteria:
Number of revertant colonies per plate

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
50+100 ug/plate +/- S9 all strains
Additional information on results:
cytotoxicity:
Experiment 1:
TA 1535, TA 1537, TA 98, TA 100, TA 102 (in absence and presence of S9 metabolic activation): 50 and/or 100 µg/plate
Experiment 2: TA 1535, TA 1537, TA 98, TA 100 (in absence and presence of S9 metabolic activation): 50 µg/plate; TA 102 (in absence and presence of S9 metabolic activation): 35 µg/plate
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test substance does not induce reverse mutation in Salmonella typhimurium under the reported experimental conditions. This information may not be used for any purpose other than in support of the Chemical safety Report submitted by Arch Chemicals Inc. under Regulation EC 1907/2006.