Lauroyl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study is comparable to OECD 403 (inhalation risk test) with acceptable restrictions mostly due to reduced reporting in times before GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

other:

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Lauroyl chloride

EC Number:

203-941-7

EC Name:

Lauroyl chloride

Cas Number:

112-16-3

Molecular formula:

C12H23ClO

IUPAC Name:

dodecanoyl chloride

Constituent 2

Reference substance name:

XVIII/195

IUPAC Name:

XVIII/195

Details on test material:

- Name of test material (as cited in study report): Laurylsaeurechlorid
- Physical state: liquid
- Purity: no data
- Expiration date of the lot/batch: not reported
- Stability under test conditions: not reported
- Storage condition of test material: not reported

Test animals

Species:

rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean weight per trial at study initiation: 171 and 167 g (mixed sexes)
no further details given

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Rats were exposed for 8 h to a vapour saturated atmosphere.
The vapour was generated by bubbling 200 l/h dry air (no CO2) through the liquid substance column (volume ca. 50 ml) of about 5 cm above a fritted glass disc in a glass cylinder. The glass cylinder was heated in a water bath. Temperature in the exposure chamber was 20°C. Nominal concentration was stated in the raw data to be 0.15 mg/L (0.156 mg/l as recalculated from the raw data).
The vapour saturation treshold is calculated to be approx. 0.21 mg/L (at 25°C). Therefor, the test atmosphere should be considered as vapour.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

Nominal concentration was 0.16 mg/L as mean of two trials with 0.11 and 0.21 mg/L, respectively

No. of animals per sex per dose:

6 (3 animals/sex in two trials)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: Weighing was performed at the start and at the end of the study. Observation was several times at the day of exposure and presumably daily on the following days except on weekends and holidays.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

none

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

> 0.16 mg/L air (nominal)

Exp. duration:

8 h

Remarks on result:

other: No mortality observed within 8 h

Mortality:

No mortality observed

Clinical signs:

other: (Slight) irritations of the mucosa, closed eye lids; reversible within 1 d

Body weight:

Mean body weight after 7 d
1st trial 192 g (weight gain +21 g); 2nd trial 183 g (+17 g)

Gross pathology:

Chronic bronchitis (2x), blown lung (1x)

Applicant's summary and conclusion

Executive summary:

Study is comparable with the inhalation hazard test described in the Annex of OECD Guideline 403 (adopted 1981) with acceptable restrictions mostly due to reduced reporting in times before GLP. Rats were exposed for 8 h to a vapour saturated atmosphere. nominal concentration was 0.16 mg/L as stated in the raw data. No mortality was reported; observed clinical signs and necropsy findings were irritation effects on the mucosa and blown lung (in one case), respectively.

Conclusion

Inhalation of 0.16 mg/L Lauroyl chloride for 8 h does not represent a lethal inhalation risk.