m-phenylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OTS 797.1400 (Fish Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

Dilution water obtained from Haskell Laboratory well with a total organic carbon content of 0.76 mg/L.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Aquatic Research Organisms in Hampton, New Hampshire
- Age at study initiation (mean and range, SD): 107 days
- Length at study initiation (mean): 3.9 cm
- Weight at study initiation (mean): 0.86 g
- Feeding during test: no food 48 hours prior or during the test

ACCLIMATION
- Acclimation conditions: in fiberglass holding tank for 37 days in continuously flowing well water (dilution water)
- Type and amount of food: frozen brine shrimp
- Feeding frequency: once or twice daily, once on Saturday, not on Sunday
- Health during acclimation (any mortality observed): no sickness, injury or abnormalities present

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

EDTA hardness:
-beginning = 76 mg/L as CaCO3
-end of test = 77 mg/L as CaCO3

Test temperature:

12.4 to 12.9 ºC

pH:

7.1 - 7.4

Dissolved oxygen:

9.6 - 10.2

Salinity:

Conductivity:
-beginning = 160 micro mhos/cm
-end of test = 160 micro mhos/cm

Nominal and measured concentrations:

Nominal test concentrations: 99, 145, 214, 314, 462, 680 and 1000 mg/L
Measured test concentrations: 107, 162, 255, 379, 552, 798 and 1108 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L glass exposure chambers
- Aeration: None
- Type of flow-through (e.g., peristaltic or proportional diluter): Mount and Brungs Proportional Diluter
- Renewal rate of test solution (frequency/flow rate): 6 times per day
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Haskell Laboratory well water
- Total organic carbon: 0.76 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark with 25 minutes of transitional light
- Light intensity: 90 lux during light and 2 lux during transitional light

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

512 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% FL 466-561 mg/L

Sublethal observations / clinical signs:

Cumulative Mortality (%)

	24 hours		48 hours		72 hours		96 hours
Measured Concentration (mg/L)	Rep 1	Rep 2	Rep 1	Rep 2	Rep 1	Rep 2	Rep 1	Rep 2
Water control	0	0	0	0	0	0	0	0
107	0	0	0	0	0	0	0	0
162	0	0	0	0	0	0	0	0
255	0	0	0	0	0	0	0	0
379	0	0	0	0	0	0	0	10
552	10	20	10	20	40	70	60	70
798	70	70	70	70	100	100	100	100
1108	100	100	100	100	100	100	100	100

Each replicate contained 10 fish.

Validity criteria fulfilled:

yes

Conclusions:

96-hour LC50: 512 mg/L

Executive summary:

The acute toxicity of the test substance to rainbow trout fingerlings in a 96 hour, flow through test was examined. Nominal concentrations ranged from 99 to 1000 mg/L. Mean, measured concentrations ranged from 107 to 1108 mg/L. The 96 hour LC50 was 512 mg/L with 95% fudicial limits of 466 and 561 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

512 mg/L