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EC number: 203-584-7 | CAS number: 108-45-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Sample storage conditions before analysis: frozen until analyzed, then thawed under room temperature
- Vehicle:
- no
- Details on test solutions:
- Test substance was prepared as a 1 g/L stock solution in distilled water and diluted in filtered laboratory mass culture well water to yield the desired nominal exposure concentrations
After mixing, 200 mL aliquots of each concentration were introduced into each of the separate 250 mL glass exposure vessels - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Haskell Laboratory-bred, raised on trout chow and yeast diet
- Age of parental stock : <24 hours
Feeding frequency during test: 3 times per week
- Test type:
- other: semi-static, renewal of test solutions 3 times per week
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
- Hardness:
- -Acute test: at the beginning = 87 mg/L as CaCO3
-Chronic test: in text states weekly, but in table only 1 value = 80 mg/L as CaCO3 - Test temperature:
- - Acute test: 20.0 - 20.1 ºC
- Chronic test: 20 +/- 1 ºC - pH:
- - Acute test: 7.1-8.3
- Chronic test: 7.1 - 8.6 - Dissolved oxygen:
- - Acute test: 3.9 - 8.5 mg/L
- Chronic test: 5.1 - 7.5 mg/L - Salinity:
- Conductivity:
-Acute test: at the beginning = 190 micromhos/cm
-Chronic test: in text states weekly, but in table only 1 value = 185 micromhos/cm - Nominal and measured concentrations:
- Nominal concentrations in mg/L: 0.1, 0.20, 0.40, 0.75, 1.50, 3.00
Measured concentrations in mg/L: nq, 0.05, 0.09, 0.29, 0.74, 2.01 - Details on test conditions:
- TEST SYSTEM - acute toxicity
- Test vessel: 250 mL glass beaker
- No aeration
- No. of organisms per vessel: 10
- H2O Controls: 2
- Photoperiod: 16 hours light: 8 hours dark
TEST SYSTEM - chronic toxicity
- Test vessel: 250 mL glass beaker
- 3 times per week, make-up water was filtered through 0.45 u Gelman filter and aerated at least 24 hours prior to use
- No. of organisms per vessel: 5 (for survival measurement), 7 (for reproduction and growth measurement)
- No. of vessels per concentration (replicates): 3 (for survival measurement), 1 (for reproduction and growth measurement)
- Photoperiod: 16 hours light: 8 hours dark
- Nominal test concentrations: 0.1, 0.2, 0.4, 0.75, 1.5, and 3.0 mg/L - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.05 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Basis for effect:
- reproduction
- Remarks on result:
- other: (0.2 mg/L nominal)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Basis for effect:
- growth
- Remarks on result:
- other: (1.5 mg/L nominal)
- Duration:
- 21 d
- Dose descriptor:
- other: MATC
- Effect conc.:
- > 0.05 - < 0.09 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Reported statistics and error estimates:
- -ANOVA and Bartlett's test used for calc. of mortality, first reproduction day, number of young, young/day, and length
-if p<0.005 for Bartlett's test, non-parametric Kruskall-Wallis and Mann-Whitney were calculated
-if p<0.005, then Dunnett's tests were calculated - Validity criteria fulfilled:
- yes
- Conclusions:
- NOEC, reproduction = 0.05 mg/L
NOEC, growth = 0.19 mg/L
MATC between 0.05 and 0.09 mg/L - Executive summary:
The objective of this study was to evaluate the chronic toxicity of the test to Daphnia magna during continuous aqueous exposure according to OECD method 211. Comparisons were made between the survival, growth and reproduction in treated and control groups. During the 21 day chronic test, reproduction was the most sensitive indicator of the toxicity followed by growth and then survival. The chronic test results concluded:
NOEC, reproduction = 0.05 mg/L
NOEC, growth = 0.19 mg/L
MATC between 0.05 and 0.09 mg/L
Reference
Chronic Toxicity Results
Measured Concentration (mg/L) |
Nominal Concentration (mg/L) |
Mortality (% Survival) |
Reproduction (young per day) |
Growth, Length (cm) |
Control blank |
Control blank |
93 |
8.8 |
3.6 |
not quantified |
0.1 |
93 |
6.4 |
3.4 |
0.05 |
0.2 |
87 |
9 |
3.6 |
0.09 |
0.4 |
60 |
6.5 |
3.4 |
0.29 |
0.75 |
87 |
5.1 |
3.6 |
0.19 |
1.5 |
100 |
2.1 |
3.2 |
2.01 |
3 |
40 |
<0.1 |
3.1 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.05 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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