Itaconic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC 84/449 B4

GLP compliance:

yes

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Itaconic acid

EC Number:

202-599-6

EC Name:

Itaconic acid

Cas Number:

97-65-4

Molecular formula:

C5H6O4

IUPAC Name:

2-methylidenebutanedioic acid

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Liver preparation from Aroclor 1254-induced rats (S9 mix)

Test concentrations with justification for top dose:

Itaconic acid was dissolved in water and dose levels used in the main assays were: 100, 500, 1000, 1500 and 2000 µg/plate without S9 mix and 100, 500, 1000, 2500 and 5000 µg/plate in presence of S9 mix.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Minimum concentration of test substance at which toxicity to bacteria was observed:

with metabolic activation : slightly toxic to TA 98 at 2500 ug/plate     

 without metabolic activation : highly toxic to TA 100 and TA 98 at 5000 ug/plate