2,2,6,6-tetramethyl-4-piperidone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 84/449/EEC C.1 Acute toxicity for fish

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,6,6-tetramethyl-4-piperidone

EC Number:

212-554-2

EC Name:

2,2,6,6-tetramethyl-4-piperidone

Cas Number:

826-36-8

Molecular formula:

C9H17NO

IUPAC Name:

2,2,6,6-tetramethylpiperidin-4-one

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples (about 150 ml in duplicate) of each test concentration were drawn from the approximate center of the test vessels. They were taken immediately before the exposure and after 96 hours exposure and kept at - 18°C to - 22°C until analysis.

Test solutions

Vehicle:

no

Details on test solutions:

Calculated amounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The fishes were then transfered into the aquaria.
The test substance was homogeneously distributed in the test vessels at all test times and test concentrations.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Supplier: West Aquarium / D-3422 Bad Lauterberg
- Number of fishes: 10 per concentration and control
- Length: 24 mm (18-28 mm)
- Weight: 0.14 g (0.07-0.21 g)
- Loading: 0.09 g/L
- Feeding: None
- Treatment: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

- Adaption: 24 hours / no food 24 hours prior to exposure
- Acclimatization: 19 days

Test conditions

Hardness:

168 mg CaCO3/L

Test temperature:

23 +/- 1°C

pH:

7.1 - 8.1

Dissolved oxygen:

85 - 100 %

Nominal and measured concentrations:

- Test concentrations (nominal): 10, 18, 32, 58 and 100 mg/L
- Measured recovery rate: 101.6 %

Details on test conditions:

- Vessels: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Water: Dechlorinated tap water (carbon filter)
- Lighting: Fluorescent light, 16 hours daily

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

63 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 52-80 mg/L

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

LC50 (24h): 76 mg/L (65-89 mg/L)
LC50 (48h): 68 mg/L (55-94 mg/L)
LC50 (72h): 65 mg/L (54-86 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions tested in an acute fish toxicity test in Danio rerio, the LD50 for fish is considered to be 63 mg/L after 96 h exposure.