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EC number: 203-825-6 | CAS number: 111-01-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,6,10,15,19,23-hexamethyltetracosane
- EC Number:
- 203-825-6
- EC Name:
- 2,6,10,15,19,23-hexamethyltetracosane
- Cas Number:
- 111-01-3
- Molecular formula:
- C30H62
- IUPAC Name:
- 2,6,10,15,19,23-hexamethyltetracosane
- Test material form:
- other: liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- other: loading rate
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
In the analyzed test medium samples from the start of the test medium renewal periods the measured test item concentration ranged between 8.4 and 1.3 μg/L. At the end of the test medium renewal periods, the test item concentration ranged between 5.4 and 1.1 μg/L. The mean measured concentration during the test period was calculated to be 3.9 μg/L. The biological results were related to the loading rate of 100 mg/L and to the mean measured test item concentration of 3.9 μg/L.
Thus, the 96-hour NOEC and LC0 of Squalane (CAS 111-01-3) to zebra fish were determined to be at least at the loading rate of 100 mg/L (mean measured 3.9 μg/L). These values might even be higher, but loading rates higher than 100 mg/L and concentrations of the test item above the solubility limit of the test item in the test water were not tested. The 96-hour LOEC, LC50 and LC100 were clearly higher than the loading rate of 100 mg/L (mean measured 3.9 μg/L). These values could not be quantified due to the absence of toxicity of Squalane (CAS 111-01-3) at the highest concentration which could be dissolved in the test water at the loading rate of 100 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the test item Squalane (CAS 111-01-3) had no acute toxic effects on the test fish up to its solubility limit in test water under the present conditions of the test.
- Executive summary:
The acute toxicity of the test item Squalane (CAS 111-01-3) to zebra fish (Danio rerio) was determined in a 96-hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/EEC, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992. A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the test organisms up to the loading rate of 100 mg/L. Due to the low water solubility of the test item, a dispersion of the test item with the loading rate of 100 mg/L was continuously stirred at room temperature in the dark over 96 hours. Then, the dispersion was filtered through a membrane filter (pore size 0.45 μm). The undiluted filtrate of the dispersion was used as the test medium. Additionally, a control was tested in parallel. A semi-static test with test medium renewal after 24 hours was performed to keep the concentration of the test item in the test medium as constant as possible during the test period of 96 hours. Since the test item was supposed to be volatile, the test was performed in a closed system as a pre-caution. The preparation of the test medium was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000. In the analyzed test medium samples from the start of the test medium renewal periods the measured test item concentration ranged between 8.4 and 1.3 μg/L. At the end of the test medium renewal periods, the test item concentration ranged between 5.4 and 1.1 μg/L. The mean measured concentration during the test period was calculated to be 3.9 μg/L. The biological results were related to the loading rate of 100 mg/L and to the mean measured test item concentration of 3.9 μg/L. In the control and at the loading rate of 100 mg/L (mean measured concentration of 3.9 μg/L), no mortality or other visible abnormalities were determined during the test period of 96 hours. In conclusion, the test item Squalane (CAS 111-01-3) had no toxic effects on zebra fish (Danio rerio) during the 96-hour test period up to a loading rate of 100 mg/L (mean measured concentration of 3.9°μg/L) respectively up to its solubility limit in the test water under the present test conditions.
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