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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable with restriction. Not according to GLP nor to specific test guidelines.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
The mutagenic activity of 61 agents as determined by the micronucleus, Salmonella, and sperm abnormality assays.
Author:
Bruce WR and Heddle JA
Year:
1979
Bibliographic source:
Can J Genet Cytol. 21(3):319-34.

Materials and methods

Principles of method if other than guideline:
The Ames Salmonella test was used to test cadmium chloride for reversion of his- auxotrophs of Salmonella Thyphimurium in the strains TA1535, TA1537, TA98 and TA100 which were obtained from Dr. B. Ames
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Cadmium chloride
EC Number:
233-296-7
EC Name:
Cadmium chloride
Cas Number:
10108-64-2
Molecular formula:
CdCl2
IUPAC Name:
cadmium(2+) dichloride
Details on test material:
name of test substance: cadmium chloride

Method

Target gene:
not applicable
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
not applicable
Additional strain / cell type characteristics:
other: each strain was irradiated at 10; 100; 1000; 10,000; 100,000 without S9
Metabolic activation:
with and without
Metabolic activation system:
S9
Test concentrations with justification for top dose:
0,05; 0,5; 5; 50; 500 µg/plate
Vehicle / solvent:
H2O
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
no
Positive control substance:
no
Remarks:
none
Details on test system and experimental conditions:
plate method of Ames (plate incorporation)
endpoint: gene mutation
Evaluation criteria:
no information
Statistics:
no statistics reported

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks:
in vitro gene mutation
Cytotoxicity / choice of top concentrations:
no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
Vehicle controls validity:
not valid
Untreated negative controls validity:
valid
Positive controls validity:
not specified
Additional information on results:
none
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

This study reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.
Executive summary:

Bruce and Heddle (1979) reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.