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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable with restriction. Not according to GLP nor to specific test guidelines.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
The mutagenic activity of 61 agents as determined by the micronucleus, Salmonella, and sperm abnormality assays.
Author:
Bruce WR and Heddle JA
Year:
1979
Bibliographic source:
Can J Genet Cytol. 21(3):319-34.

Materials and methods

Principles of method if other than guideline:
The Ames Salmonella test was used to test cadmium chloride for reversion of his- auxotrophs of Salmonella Thyphimurium in the strains TA1535, TA1537, TA98 and TA100 which were obtained from Dr. B. Ames
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
name of test substance: cadmium chloride

Method

Target gene:
not applicable
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
not applicable
Additional strain / cell type characteristics:
other: each strain was irradiated at 10; 100; 1000; 10,000; 100,000 without S9
Metabolic activation:
with and without
Metabolic activation system:
S9
Test concentrations with justification for top dose:
0,05; 0,5; 5; 50; 500 µg/plate
Vehicle / solvent:
H2O
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
no
Positive control substance:
no
Remarks:
none
Details on test system and experimental conditions:
plate method of Ames (plate incorporation)
endpoint: gene mutation
Evaluation criteria:
no information
Statistics:
no statistics reported

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks:
in vitro gene mutation
Cytotoxicity / choice of top concentrations:
no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
Vehicle controls validity:
not valid
Untreated negative controls validity:
valid
Positive controls validity:
not specified
Additional information on results:
none
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

This study reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.
Executive summary:

Bruce and Heddle (1979) reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.