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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2011-12-16 to 2013-03-15
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with international standard guidelines under GLP conditions. The study report was well documented with all mandatory information included.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
2012-11-30

Test material

Constituent 1
Chemical structure
Reference substance name:
Octylphosphonic acid
EC Number:
225-218-5
EC Name:
Octylphosphonic acid
Cas Number:
4724-48-5
Molecular formula:
C8H19O3P
IUPAC Name:
octylphosphonic acid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Water samples were taken from the control and each test group (Replicates R1-R4 pooled) at 0 end 48 hours for quantitative analysis. All samples were stored at approximately -20°C prior to analysis. Duplicate samples were taken and stored at approximately -20°C for further analysis if necessary.
Samples at the NOEC and above only were analyzed.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:Daphnia magna
- Strain:
- Source: In-house laboratory culture
- Age at study initiation (mean and range, SD): less than 24 hours
- Method of breeding: Culture conditions ensured that reprodcution was by pathenogenesis
- Feeding during test: No
- Food type: Algal suspension (Desmodesmus subspicatus and tetramin® flake food suspension
- Amount: No data
- Frequency: Daily

ACCLIMATION
- Acclimation period: No

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
No data
Test temperature:
18°C to 23°C (see table Physico-chemical measurements)
pH:
7.1 to 8 (see table Physico-chemical measurements)
Dissolved oxygen:
99% to 101% ASV (Air saturation value)
Salinity:
No applicable
Nominal and measured concentrations:
Nominal concentration: 0, 1.0, 1.8, 3.2, 5.6, 10, 18, 32 mg/L
Measured concentration: Started for the 3.2 mg/L concentration: 3.18, 5.80, 10.2, 18.3, 27.3 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jar
- Type (delete if not applicable): covered to prevent evaporation
- Material, size, headspace, fill volume: 250mL filled at 200 mL
- Aeration:No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
See paragraph any other information on material and methods incl. tables

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 light and 8 hours darkness with 20 minutes dawn ans dusk transition periods
- Light intensity: 751-791 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization at 24h and 48h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: The substance was not soluble in the medium above 32 mg/L
- Range finding study: Yes
- Test concentrations: 0.1; 1.0; 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Immobilisation showed at 10 and 100 mg/L, and limit of solubility of the substance at 32 mg/L
Reference substance (positive control):
yes
Remarks:
*potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities:
- Other biological observations: Microscopic examination of the immobilized daphnids after 48-hours exposure showed that at the concentration of 5.6 mf/L and above, particle of test item were adhered to the antenna and tail.
- Mortality of control: No
- Other adverse effects control:No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The 32 mg/L test prapration was observed to be slighly cloudy dispersion with particles of test item floating at the water surface at the start of the test. After both 24h and 48h exposure, the preparation was observed to have formed a slightly cloudy dispersion with a layer of test item at the water surface.
- Effect concentrations exceeding solubility of substance in test medium: Significant precipitation was observed to occur at concentration in excess of 32 mg/L
Results with reference substance (positive control):
- Results with reference substance valid?
- Mortality: yes
- EC50/LC50: 0.45 mg/L (0.42-0.48 mg/L at 95% confidences limits

Any other information on results incl. tables

Table2: Cumulative immobilization data in the definitive test

Nominal concentration (mg/L)

Cumulative immobilized Daphnia (Initial pop. = 5 per replicate)

24 hours

48 hours

R1

R2

R3

R4

Total

%

R1

R2

R3

R4

Total

%

Control

0

0

0

0

0

0

0

0

0

0

0

0

1.0

0

0

0

0

0

0

0

0

0

0

0

0

1.8

0

0

0

0

0

0

0

0

0

0

0

0

3.2

0

0

0

0

0

0

0

1

0

0

*1

5

5.6

0

0

0

0

0

0

0

1

0

1

2

10

10

0

0

0

0

0

0

2

1

0

0

3

15

18

1

0

0

1

2

10

1

0

1

1

3

15

32

0

0

0

0

0

0

1

0

2

3

6

30

 

R1-R4 = Replicate 1 to 4

* = Single immobilized daphnid considered to be due to natural causes rather than a true toxic response given that less than 10% immobilization occurred.

Analytical results for test samples

Time point

(h)

Nominal concentration of test item in test sample

Cnom

[mg/L]

Measured concentration of test item in sample vial

X

[mg/L]

Sample preprataion factor

 

F

Dtermined concentration of test item in test sample

C

[mg/L]

% of nominal concentration

 

 

 

[%]

0

Control

< LOQ

1

< LOQ

1

3.2

0.636

5

3.18

99

5.6

0.580

10

5.80

104

10

0.510

20

10.2

102

18

0.458

40

18.3

102

32

0.546

50

27.3

85

48

Control

< LOQ

-

< LOQ

-

3.2

0.650

5

3.25

102

5.6

0.613

10

3.13

109

10

0.520

20

10.4

104

18

0.435

40

17.4

97

32

0.534

50

26.7

83

LOQ = Limit Of Quantification

- = Not applicable

For reporting purpose, X has been calculated from C retrospectively

Physico-Chemical measurements

Nominal concentration (mg/L)

 

0 Hours

 

 

24 hours

48 hours

 

 

 

 

 

 

pH

Mg O2/L

% ASV*

T°C

T°C

pH

Mg O2/L

% ASV*

T°C

Control

R1

8.0

8.9

100

21

22

7.6

8.7

98

21

R2

8.0

8.9

100

21

21

7.6

8.7

98

21

R3

7.9

8.9

100

21

22

7.7

8.7

98

21

R4

7.9

8.9

100

21

21

7.7

8.7

98

21

1.0

R1

7.9

8.9

100

21

22

7.7

8.7

98

21

R2

7.9

8.9

100

21

21

7.7

8.7

98

21

R3

7.9

8.9

100

21

22

7.7

8.7

98

21

R4

7.9

8.8

99

21

21

7.7

8.7

98

21

1.8

R1

7.9

8.8

99

21

22

7.7

8.7

98

21

R2

7.9

8.8

99

21

21

7.7

8.7

98

21

R3

7.9

8.8

99

21

22

7.7

8.7

98

21

R4

7.8

8.8

99

21

21

7.7

8.7

98

21

3.2

R1

7.8

8.8

99

21

22

7.7

8.7

98

21

R2

7.8

8.8

99

21

21

7.7

8.7

98

21

R3

7.8

8.8

99

21

22

7.7

8.7

98

21

R4

7.8

8.8

99

21

21

7.7

8.7

98

21

5.6

R1

7.7

8.8

99

21

22

7.7

8.7

98

21

R2

7.7

8.8

99

21

21

7.7

8.7

98

21

R3

7.7

8.8

99

21

22

7.7

8.7

98

21

R4

7.7

8.8

99

21

21

7.7

8.7

98

21

10

R1

7.6

8.8

101

22

22

7.7

8.7

98

21

R2

7.6

8.8

101

22

21

7.6

8.7

98

21

R3

7.6

8.7

100

22

22

7.6

8.7

98

21

R4

7.5

8.7

100

22

21

7.6

8.7

98

21

18

R1

7.4

8.7

100

22

22

7.6

8.7

98

21

R2

7.4

8.7

100

22

21

7.6

8.7

98

21

R3

7.3

8.7

100

22

22

7.6

8.7

98

21

R4

7.3

8.7

100

22

21

7.5

8.7

98

21

32

R1

7.2

8.7

100

22

21

7.5

8.7

98

21

R2

7.1

8.6

99

22

21

7.4

8.7

98

21

R3

7.1

8.6

100

23

22

7.4

8.7

98

21

R4

7.1

8.6

100

23

22

7.4

8.7

98

21

* ASV = Dissolved oxygen concentration expressed as a percentage of Air Saturation Value

R1 – R4 = Replicate 1 to 4

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of the test item to the freshwater invertebrate (Daphnia Magna) has been investigated and gave a 48-hours EC50 of greater than 32 mg/L. The NOEC was 3.2 mg/L.

Due to the limited solubility of the test item in the test media, it was considered to be inappropriate to test at concentrations in excess of 32 mg/L. significant precipitation was observed to occur at concentrations in excess of 32 mg/L which may have exerted physical effects on the test organism wich were unrelated to toxicity. Microscopic examination of the immobilized daphnids after 48 hours exposure showed that the concentration of 5.6 mg/L and above, particles of test item were adhered to the antenna and tail. As such it was not possible to ascertain whether the immobilization observed was due to either a physical and/or a toxicological effect.
Executive summary:

The 48–hr-acute toxicity of OPA to Daphnia magna was studied under static conditions.  Daphnids were exposed to control and test chemical at nominal concentration of 1.0, 1.8, 3.2, 5.6, 10, 18 and 32 mg/L for 48 hr.  Immobility were observed daily. 

The 48– hour EC50was > 32 mg/L. 

The test was not carried at a concentration above than 32 mg/L because it was not possible to solubilize the subtance at a level upper than 32 mg/L in the medium (observations made during the range-finding study)

 

It satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Results Synopsis

 Test Organism :Daphnia magna

 NOEC:  3.2 mg.//L   

EC50: >32 mg/L