p-(1,1-dimethylpropyl)phenol

Long-term toxicity to fish

Description of key information

 A study (Seki et al. 2003) provides an estimated NOEC of 0.10 mg/L based on fertility/reproduction.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.1 mg/L

Additional information

Reliable (Klimisch 1 or 2) long-term toxicity studies with fish have been conducted for p-tert-amylphenol and are included in this dossier.

The long-term toxicity of p-tert-amylphenol to fish was investigated by Seki et al. (2003) (Klimisch 1). The fish, Medaka (Oryzias latipes), was exposed to the test chemical for two generations following methods similar to OECD 210. The flow-through test was initiated with eyed-eggs and hatchability and time to hatch was determined and no significant difference from the control was found for the F0 generation. Growth, fecundity and fertility of the F0 generation were also determined. For the F0 generation, the NOEC for growth, fecundity, and fertility was 0.402 mg/L, 0.931 mg/L and 0.100 mg/L, respectively. For the F1 generation, the NOEC for growth was 0.100 mg/L.

Panter et al. (2006) conducted a study (Klimisch 1) with p-tert-amylphenol. Embryos (24hr post fertilization) of fathead minnow (Pimephales promelas) were exposed to test substance in a flow-through system in measured concentrations of 0.056, 0.18 and 0.56 mg/L for 107 days post hatch following methods based on OECD 210. Although the focus of this study was to measure effects on the gonads, secondary sexual characteristics and vitellogenin, survival and growth were also assessed. The NOEC at 107 days post hatch, based on survival and growth were 0.56 and 0.56 mg/L. respectively. The lowest NOEC of 0.18 mg/L was based on gonadosomatic index and gonadal sex of fish.

Gimeno et al. (1998) conducted a study (Klimisch 3) with p-tert-amylphenol. Male larvae, 50 days post-hatch, of common carp (Cyprinus carpio), were exposed to test substance in an intermittent flow-through system in measured concentrations of 0.10, 0.32 and 1 mg/L for 110 days. Although the focus of this study was to measure effects on the reproductive tract development, secondary sexual characteristics and vitellogenin, growth was also assessed. The NOEC, based on growth was >0.256 mg/L. Although this study is deemed not reliable since it did not follow acceptable guidelines, it is presented here for weight of evidence support.

Reliable (Klimisch 1) long-term toxicity studies with fish have been conducted for p-tert-amylphenol and are included in this dossier. A study (Seki et al. 2003) provides an estimated NOEC of 0.10 mg/L based on fertility/reproduction. Consequently this value will be taken as the long-term toxicity in fish endpoint for p-tert-amylphenol.