Amines, C16-18-alkyl

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Amines, tallow alkyl

EC Number:

263-125-1

EC Name:

Amines, tallow alkyl

Cas Number:

61790-33-8

IUPAC Name:

61790-33-8

Constituent 2

Reference substance name:

Amines, tallow alkyl

IUPAC Name:

Amines, tallow alkyl

Details on test material:

- Name of test material (as cited in study report): Genamin TA 100 D

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

The test substance was weighed out on quartz sand.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: own breeding, original source was the Bundesanstalt für Land und Forstwirtschaft.
- Weight at test initiation: 0.30 - 0.60 g

Housing:
- Housing conditions: Organisms were bred at the testing facility in covered plasitc vessels (volume 60 L) containing potting compost at 20 ± 2°C in the dark

ACCLIMATION
- Acclimation period: 1 day

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 ± 2 °C

Details on test conditions:

TEST SYSTEM
- Test container (material, size): glass dishes, 1 L volume
- Amount of soil or substrate:
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate):
10% peat
20% kaolin
70 % quartz sand
0.4 % calcium carbonate
- Organic carbon (%):
- Pretreatment of soil: deionised water was added to give a overall moisture content of about 25 % of dry weight. The definitive moisture content, determinated the day before application by drying a sample at 105 °C and re-weighing, was 16.3 %.



OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 400/800 lux

EFFECT PARAMETERS MEASURED : mortality and weight

Nominal and measured concentrations:

Nominal concatrations: control, 100, 180, 320, 580, 1000 mg/kg dry weight.

Reference substance (positive control):

yes

Remarks:

2-chloracetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

8 wk

Dose descriptor:

LC10

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

8 wk

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: LC 50 (14d) = 42.1 mg/kg

Any other information on results incl. tables

No mortality or singificant pathological symptoms were seen at the concentrations 100 -1000 mg/kg.