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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, limited no. of strains used

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity of inhalation anaesthetics: trichloroethylene, divinyl ether, nitrous oxide and cyclopropane
Author:
Baden J.M., Kelley M., Mazze R.I., & Simmon, V.F.
Year:
1979
Bibliographic source:
Br. J. Anaesth. 51; pp 417-421

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
limited no. of strains used
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Dinitrogen oxide
EC Number:
233-032-0
EC Name:
Dinitrogen oxide
Cas Number:
10024-97-2
Molecular formula:
N2O
IUPAC Name:
Dinitrogen Oxide
Test material form:
gas under pressure: liquefied gas

Method

Species / strain
Species / strain / cell type:
other: S. typhimurium TA 1535, TA 100
Additional strain / cell type characteristics:
not applicable
Metabolic activation:
with and without
Metabolic activation system:
Aroclor 1254
Test concentrations with justification for top dose:
0, 1, 3, 9, 27, 81%
Controls
Untreated negative controls:
yes
Positive controls:
yes
Positive control substance:
other: vinylidene chloride, 2-anthramine

Results and discussion

Test results
Species / strain:
other: S. typhimurium TA 1535, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
not applicable
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

No increase in the number of revertant colonies were observed in strain TA100 or TA1535. At the maximum dose tested, 81%, no evidence of cytotoxicity was observed.