Bis(2-dimethylaminoethyl)(methyl)amine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study, that meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Principles of method if other than guideline:

5 mice per sex per dose were administered a single dose of the test substance via intraperitoneal injection. Clinical signs were monitored and necropsy was carried out at termination or after death if an animal died during the study. Observation period was until day 7.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2-dimethylaminoethyl)(methyl)amine

EC Number:

221-201-1

EC Name:

Bis(2-dimethylaminoethyl)(methyl)amine

Cas Number:

3030-47-5

Molecular formula:

C9H23N3

IUPAC Name:

(2-{[2-(dimethylamino)ethyl](methyl)amino}ethyl)dimethylamine

Details on test material:

- Name of test substance (as cited in study report): Pentamethyl-diaethylen-trieamin
- Analytical purity: no data
- Physical state: liquid
- Density: 0.829 g/cm³
- Substance number: XXIII/80

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Source: Kisslegg
- Mean weight at study inititation: 23.5 g (males), 21.4 g (females)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

1600, 800, 400, 360, 320, 250 and 200 µL/kg bw (density: 0.829 g/cm³)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 315 mg/kg bw

Based on:

test mat.

95% CL:

> 298 - < 332

Remarks on result:

other: Corresponds to ca. 380 µL/kg bw; Conversion based on a density of 0.829 g/cm³.

Mortality:

- 1600 and 800 µL/kg bw: 10/10 animals died
- 400 µL/kg bw: 9/10 animals died
- 360 µL/kg bw: 3/10 animals died
- 320, 250 and 200 µL/kg bw: 0/10 animals died
(for details see table below)

Clinical signs:

- 1600 µL/kg bw: increased respiration, spastic gait, clonic convulsions, dyspnoea (animals died within 10 min)
- 800 and 400 µL/kg bw: increased respiration, spastic gait, atonia, apathy. surviving animals from day 5 without symptoms
- 400 - 200 µL/kg bw increased respiration, spastic gait, tremor, convulsions, crouched position. Surviving animals were without symptoms after 2 - 7 days.

Body weight:

Body weight range study initiation:
males: 17 g - 34 g
females: 17 g - 32 g

Any other information on results incl. tables

Mortality:

Dose (µL/kg )	Conc.(%)	Gender	1 h	24 h	48 h	day 7
1600	16	male	5/5	5/5	5/5	5/5
	16	female	5/5	5/5	5/5	5/5
800	4	male	0/5	5/5	5/5	5/5
	4	female	0/5	5/5	5/5	5/5
400	4	male	0/5	4/5	5/5	5/5
	4	female	0/5	3/5	3/5	4/5
400	2	male	0/5	3/5	3/5	5/5
	2	female	0/5	1/5	1/5	3/5
360	2	male	0/5	0/5	2/5	3/5
	2	female	0/5	0/5	0/5	0/5
320	2	male	0/5	0/5	0/5	0/5
	2	female	0/5	0/5	0/5	0/5
250	2	male	0/5	0/5	0/5	0/5
	2	female	0/5	0/5	0/5	0/5
200	2	male	0/5	0/5	0/5	0/5
	2	female	0/5	0/5	0/5	0/5