Registration Dossier
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EC number: 221-424-4 | CAS number: 3089-17-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 17 JUL 1992 to 31 JUL 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (EPA TSCA, 40 CFR 798)
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA TSCA, 40 CFR 798
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2,9-dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 221-424-4
- EC Name:
- 2,9-dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Cas Number:
- 3089-17-6
- Molecular formula:
- C20H10Cl2N2O2
- IUPAC Name:
- 2,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Ave Animals
- Age at study initiation: ~8 weeks
- Weight at study initiation: males: 257 - 278 g; females: 205 - 248 g
- Fasting period before study: 16 to 17 h
- Housing: in cages in groups of five by sex
- Diet: Fresh Purina Rat Chow (Diet #5012), ad libitum
- Water, ad libitum
- Acclimation period: at least one week
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0.5 g/mL (20 g of test article was added to corn oil for total volume of 40 mL)
MAXIMUM DOSE VOLUME APPLIED: 1 mL/100 g body weight for non-aqueous vehicles and 2 mL/100 g body weight for aqueous solutions - Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: 1, 2 and 4 hours post dose and once daily for 14 days toxicity and pharmacological effects, twice daily for 14 days for mortality
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes - Statistics:
- The LD50, 95% Confidence limits, dose response curve and slope were calculated, if possible, according to the method of Litchfield and Wilcoxon, 1949 or Horn, 1956.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Remarks on result:
- other: no animals died within the 14 days observation period
- Mortality:
- - no deaths occurred
- Clinical signs:
- - one animal had diarrhea 4 hours post appilcation
- in all animals the anogenital area was stained purple on days 1 and 2 - Body weight:
- - body weight development was not impaired
- Gross pathology:
- - all animals were sacrificed at the end
- only in one male animal there was a herniated liver protruding through the diaphragm
- in all other animals no macroscopically visible changes were found
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: other: CLP regulation
- Conclusions:
- Single application of the limit dose of 5000 mg test item per kg bw did not cause lethality in male and female Wistar rats during the 14 days observation period, resulting in a LD50 > 5000 mg/kg bw.
- Executive summary:
Acute oral toxicity of the test item was tested in male and female Wistar rats according to EPA TSCA, 40 CFR 798. The test was conducted administering the limit dose of 5000 mg/kg bw to 5 animals/sex. During the 14 days observation period no animals died and there were no toxicological relevant changes found in necropsy, thus leading to a median lethal dose (LD50) > 5000 mg/kg bw.
Therefore, the test item has not to be classified for acute oral toxicity according to Regulation (EC) No 1272/2008.
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