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EC number: 200-289-5 | CAS number: 56-81-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
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- Flash point
- Auto flammability
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- Explosiveness
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted prior to GLP and test guidelines, but sufficient data is available for interpretation of results
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 938
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Test material was administered dermally to groups of guinea pigs. The material remained in contact for 4 days. Animals were observed for two weeks removal of the dermal bandage.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Glycerol
- EC Number:
- 200-289-5
- EC Name:
- Glycerol
- Cas Number:
- 56-81-5
- Molecular formula:
- C3H8O3
- IUPAC Name:
- propane-1,2,3-triol
- Details on test material:
- No additional information available.
Constituent 1
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No additional information available.
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- The hair was removed with electric clippers from a band of skin about three inches wide, completely encircling the animal between the fore and hind legs. One day was then allowed for healing of any small accidental skin nicks. Then the animal was weighed and the dose calculated in terms of millitliters of material per kilo body weight. A piece of three-inch adhesive tape was cut, long enough to encircle the clipped area about twice. On this a flat pad of absorbent cotton was laid, thick enough and large enough to retain the entire calculated dose. For the larger doses, this cotton pad was about 2 x 10 cm., and the full thickness of cotton in a standard one pound roll. For smaller doses the pad was made proportionately smaller and thinner. This cotton pad then received the measured dose and the tape and pad were wrapped about the pig, covering the clipped area, the cotton over the abdomen. About three, or in most cases four days, the tape was removed without the use of solvents, the skin area observed for signs of irritation other than that due to the tape.
- Duration of exposure:
- three or four days
- Doses:
- 16, 40, 50 and 60 ml/kg
- No. of animals per sex per dose:
- 5 guiinea pigs (mixed sex) were dosed at 50 and 60 ml/kg, 8 guinea pigs were dosed at 40 ml/kg and 1 guinea pig was dosed at 16 ml/kg. One male was dosed with 16 ml/kg, 3 males and 5 females were dosed with 40 ml/kg, 1 male and 4 females were dosed with 50 ml/kg and 4 males and 1 female were dosed with 60 ml/kg.
- Control animals:
- not specified
- Details on study design:
- Following removal of the bandage the animals were kept under observation for two weeks, or until they died.
- Statistics:
- No additional information available.
Results and discussion
- Preliminary study:
- Not applicable.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 45 mL/kg bw
- Based on:
- test mat.
- Mortality:
- See Table below
- Clinical signs:
- other: After about 12 hours the animals became accustomed to the restriction of the bandage and ate and in general behaved as usual, those having large doses being weak with low body temperature and dying; those with small doses apparently not being affected.
- Gross pathology:
- No additional information available.
- Other findings:
- No additional information available.
Any other information on results incl. tables
Mortality
Dose Level (ml/kg) | Number dosed | Number survived |
16 | 1 | 0 |
40 | 8 | 3 |
50 | 5 | 4 |
60 | 5 | 3 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute dermal toxicity of glycerin was examined in guinea pigs. The dermal LD50 was determined to be 45 ml/kg (56,750 mg/kg) in guinea pigs.
- Executive summary:
The acute dermal toxicity of glycerin was examined in guinea pigs. The dermal LD50 was determined to be 45 ml/kg (56,750 mg/kg) in guinea pigs.
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