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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP status unknown, results are described well and adequate for assessment, published in peer-reviewed literature
Principles of method if other than guideline:
Near-guideline test
GLP compliance:
not specified
Analytical monitoring:
yes
Details on sampling:
Water samples were analyzed 5 days per week throughout the 28-day exposure period; sample collection was from the mid-point of the tanks.
Vehicle:
yes
Test organisms (species):
Jordanella floridae
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d
Hardness:
48 mg/l as CaCO3
Test temperature:
25 oC
pH:
6.95
Reference substance (positive control):
not specified
Duration:
10 d
Dose descriptor:
NOEC
Effect conc.:
5.76 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Standard deviation: 0.77
Duration:
28 d
Dose descriptor:
other: MATC
Effect conc.:
> 21.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
number hatched
Duration:
10 d
Dose descriptor:
other: MATC
Effect conc.:
11 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
other: MATC
Effect conc.:
14.85 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
other: MATC
Effect conc.:
> 20.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate

Maximum acceptable toxicant concentrations (MATC) were calculated (MATC is the geometric mean of the NOEC and LOEC) for each endpoint:

Egg hatchability MATC > 21.2 mg/l

10-day larval survival MATC = 11.0 mg/l

28-day fry survival MATC = 14.85 mg/l

28-day fry growth MATC > 20.9 mg/l

The most sensitive of the endpoints studied was larval survival over ten days, which resulted in a NOEC of 5.76 ± 0.77 mg/l.

Description of key information

A 10-day NOEC of 5.76 mg/l and a 28-day MATC of 14.85 mg/l are reported for the larvae and fry of Jordanella floridae.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
5.76 mg/L

Additional information

A study similar to early life stage (ELS) test was available (Smith et al. 1991).  The study had a flow-through system, the concentrations were analysed 5 days per week throughout 28-day exposure period.  Two stages of American flagfish (Jordanella floridae) were used in simultaneous flow-through exposures: embryo-larval fish (hatching success and 10-day larval survival) and fry fish (survival and growth over 28 days).  The lowest NOEC was for 10-day larval survival, at 5.76 mg/l and this value was used in the assessment.  Another study was available from Loekle et al. (1983), but this study was not considered reliable as long time periods occurred between solution renewals, the concentrations were not monitored and the endpoint was not clearly defined.