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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non-guideline study, some restrictions in reporting, nevertheless acceptable for assessment.

Data source

Reference
Reference Type:
publication
Title:
Effects on experimental animals of acute, repeated and continuous inhalation exposures to dichloroacetylene mixtures.
Author:
Siegel J, Jones RA, Coon RA, Lyon JP.
Year:
1971
Bibliographic source:
Toxicol Appl Pharmacol. 1971 Jan;18(1):168-74.

Materials and methods

Principles of method if other than guideline:
Determination of the LC50
GLP compliance:
no
Test type:
acute toxic class method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Trichloroethylene
EC Number:
201-167-4
EC Name:
Trichloroethylene
Cas Number:
79-01-6
Molecular formula:
C2HCl3
IUPAC Name:
1,1,2-trichloroethene
Constituent 2
Reference substance name:
trichloroethene
IUPAC Name:
trichloroethene
Details on test material:
The substance was obtained commercially. No other details were reported.

Test animals

Species:
rat
Strain:
other: NMRI:O(SD) Sprague-Dawley derived
Sex:
male
Details on test animals or test system and environmental conditions:
No details were reported.

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
not specified
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Vapor of TCE was obtained in high concentrations by passing air through a large fritted gas absorber containing the material and then collected in a metallized plastic bag. All exposures were conducted under a fume hood. The animals were exposed for 4 hours and then returned to their individual cages for a 2-week observation period. No food or water was provided during the exposure.
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Gas chromatography equipped with a hydrogen flame ionisation detector. The chromatograph was equipped with an automatic valve which permitted sampling every 5 min during the exposure periods.
Duration of exposure:
4 h
Concentrations:
6750, 8000 and 14700 ppm.
No. of animals per sex per dose:
16
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: no data
Statistics:
Not reported.

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
12 500 ppm
Exp. duration:
4 h
Remarks on result:
other: Estimated by the method of Miller and Tainter (1944)
Mortality:
Mortality rate:
6750 ppm: 2/16
8000 ppm: 3/16
14700 ppm: 10/16

All deaths occurred during exposure.
Clinical signs:
other: Not reported.
Body weight:
Not reported.
Gross pathology:
Not reported.
Other findings:
Not reported.

Applicant's summary and conclusion