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EC number: 214-946-9 | CAS number: 1222-05-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24/09/1996 -25/08/1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliability 2 is assigned because the test has been carried out following internation guideling and under GLP without deviations that influence the results, however, the tested concentrations were not verified with expertimental analysis
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
- Deviations:
- yes
- Remarks:
- : ISO/CD 11267 ISO Draft Soil Quality: Effects of soil pollutants on Collembola (Folsomia candida): Mathod for the determination of effects on reproduction (Version 3/96)
- GLP compliance:
- yes (incl. QA statement)
- Application method:
- soil
Test material
- Reference substance name:
- 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylindeno[5,6-c]pyran
- EC Number:
- 214-946-9
- EC Name:
- 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylindeno[5,6-c]pyran
- Cas Number:
- 1222-05-5
- Molecular formula:
- C18H26O
- IUPAC Name:
- 4,6,6,7,8,8-hexamethyl-1H,3H,4H,6H,7H,8H-indeno[5,6-c]pyran
- Details on test material:
- Batch # 7953-I
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Samples were taken at the start of the test (7 days after application), first day of exposure and at the end of the study.
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Acetone was used as a solvent.
Test organisms
- Test organisms (species):
- Folsomia candida
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- The test was started with juvenile springtails of the species Folsomia candida 10 to 12 days of age and survival and reproduction after 28 days were determined.
Study design
- Study type:
- laboratory study
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Post exposure observation period:
- No data
Test conditions
- Test temperature:
- 17 - 25 degrees Celsius
- pH (if soil or dung study):
- at start of study pH 6.1 - 6.4, after 4 weeks pH 5.7 - 5.9
- Humidity:
- water content adjusted to 40-60% of total water holding capacity, corresponding to approx. 30% of dry weight.
Water content at start of study: 33.6 - 34.9 % of dry weight, after 4 weeks: 26.6 - 30.6 % of dry weight - Photoperiod and lighting:
- dark/light cycle 8h/16h, 400 - 530 lux
- Details on test conditions:
- Composition artifical soil: 10% sphagnum-peat; 20% kaolinite clay;circa 0.5% CaCO3 to adjust pH to 6.0+/- 0.5; approx. 50.1% fine quartz sand (grain size 0.1 - 0.5 mm). Solvent: acetone; test range: 1 - 105 mg/kg.
Animals were fed with granulated dry yeast, added during the test when necessary.
The test materials at appropriate concentrations were dissolved in equal amounts of acetone, mixed with the quartz sand and allowed to slowly evaporate and mixed with the standard (DIN) soil. After preparation of the test concentrations and an equilibrium period of one week, the test organisms were added to the soil. The test medium was not refreshed during the test period.
10 springtails per test container. Test containers were covered with a lid to prevent excessive evaporation. Opening for 10 seconds twice a week for aeration. Water (after weighing) and food added when needed. - Nominal and measured concentrations:
- Nominal test concentrations were 1, 3, 8, 19, 45 and 105 mg/kg soil. Samples were taken in case analyses would be considered to be necessary. No analyses were carried out.
- Reference substance (positive control):
- yes
- Remarks:
- Betanal (CQ 532); active ingredient 160g/l Phenmdeipham. Applied 200 mg Betanal/kg soil.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 4 wk
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 105 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- No significant mortality was observed in soils containing up to 45 mg/kg, but mortality was 72% in 105 mg/kg. Reproduction was inhibited by 23% in 45 mg/kg. However, this inhibition was not statistically significantly different from the control. The reproduction in 105 mg/kg was 16% of the control. Thus, the NOEC for HHCB is 45 mg/kg based on survival
- Results with reference substance (positive control):
- No mortality was observed for the reference compound and the reproduction was 56.3% of the controls.
- Reported statistics and error estimates:
- Mortality: Significance of acute toxic effects were tested with Mann&Whitney U-test (p = 0.05); effects at a dosage of 105mg/kgdw (artificial soil) differed significantly from the control.
Reproduction: reproduction rates at the highest dose tested (105 mg/kgdw) were significantly reduced (by 84%) to the control (Dunnett test, p = 0.05)
Any other information on results incl. tables
Table - Mortality of adult springtails per replicate (mean per treatment, standard deviation, n = 5)
Treatment | Concentration (mg/kgdw) | Mean (% of initial number)) | Std.dev. (%) |
control | 0 | 0.0 | 0.0 |
solvent control | 0 | 2.0 | 3.3 |
C1 | 1.0 | 0.0 | 0.0 |
C2 | 3.0 | 4.0 | 5.0 |
C3 | 8.0 | 0.0 | 0.0 |
C4 | 19.0 | 6.0 | 4.4 |
C5 | 45.0 | 4.0 | 4.4 |
C6 | 105.0 | 72.0 | 25.9 |
reference compound |
| 0.0 | 0.0 |
Table - Reproduction: number of juvenile springtails per replicate (mean per treatment, standard deviation, n = 5)
Treatment | Concentration (mg/kgdw) | Mean | Std.dev. |
control | 0 | 395 |
|
solvent control | 0 | 338 | 82 |
C1 | 1.0 | 466 | 72 |
C2 | 3.0 | 409 | 101 |
C3 | 8.0 | 380 | 119 |
C4 | 19.0 | 490 | 83 |
C5 | 45.0 | 284 | 87 |
C6 | 105.0 | 59 | 17 |
reference compound |
| 207 | 55 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality was 1% (required < 20%); mean reproduction rate 367 per control vessel (required at least 100), with coeff. of var. 22% (required < 30%).
- Conclusions:
- 4wk-NOEC = 45 mg/kg dwt; LOEC = 105 mg/kg dwt.
- Executive summary:
The springtail Folsomia candida was tested according to the draft ISO/CD 11267. The test materials at appropriate concentrations were dissolved in equal amounts of acetone, mixed with the quartz sand and allowed to slowly evaporate and mixed with the standard soil containing 10% Sphagnum peat, 20% kaolinite clay, approximately 70% fine quartz-sand (grain size 0.1-0.5 mm) and 0.5% calcium carbonate to adjust to pH 6.0±0.5. After preparation of the test concentrations and an equilibrium period of one week, the test organisms were added to the soil. The test medium was not refreshed during the test period.
The test was started with juvenile springtails of the species Folsomia candida 10 to 12 days of age and survival and reproduction after 28 days were determined. Nominal test concentrations were 1, 3, 8, 19, 45 and 105 mg/kg soil. The animals were fed with granulated dry yeast. No significant mortality was observed in soils containing up to 45 mg/kg, but mortality was 72% in 105 mg/kg. Reproduction was inhibited by 23% in 45 mg/kg. However, this inhibition was not statistically significantly different from the control. The reproduction in 105 mg/kg was 16% of the control. Thus, the NOEC for HHCB is 45 mg/kg based on survival.
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