N-(cyclohexylthio)phthalimide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(cyclohexylthio)phthalimide

EC Number:

241-774-1

EC Name:

N-(cyclohexylthio)phthalimide

Cas Number:

17796-82-6

Molecular formula:

C14H15NO2S

IUPAC Name:

N-(cyclohexylthio)phthalimide

Details on test material:

Batch No.: 093/06
Purity: 99.43% ( w/w)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

volume of test substance solution: 50 mL ( least 2 mL per individual)
Content of (Ca+Mg) ions in dilution water: 2.59 - 2.66mmol/L
pH of dilution water: 7.8 - 7.9

Preliminary test:
stock solution of test substance: 50.09 mg/0.5L saturated solution in dilution water determined concentration: 9.16 mg/L
saturated solution dosing: 1000, 500, 100, 50, 10 mL in 1000mL of dilution water
conductivity of distiled water: 0.153 mS/m
pH of dilution water: 7.9
Oxygen content: 7.2 mg/L at 19.9°C
content of (Ca+Mg) in dilution water: 2.66 mmol/L

Full semi-static test:
Test solution 1: Stock solution of test substance: 100.04 mg/L saturated solution in dilution water , determined concentration : 4.48 mg/L
saturated solution dosing: 1000, 870, 760, 660, 570, 500, 430 mL in 1000 mL of dilution water.
conductivity of distiled water: 0.119 mS/m
pH of dilution water: 7.9
Oxygen content: 9.0 mg/L at 19.9°C
content of (Ca+Mg) in dilution water: 2.61 mmol/L
Test solution 2 ( renewal the test solution after 24 hours): Stock solution of test substance: 100.07 mg/L saturated solution in dilution water , determined concentration : 4.25 mg/L
saturated solution dosing: 1000, 870, 760, 660, 570, 500, 430 mL in 1000 mL of dilution water.
conductivity of distiled water: 0.119 mS/m
pH of dilution water: 7.8
Oxygen content: 8.9 mg/L at 19.7°C
content of (Ca+Mg) in dilution water: 2.59 mmol/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mxture of algae. The sensitivity of test species is periodically monitored by testing with the reference substance, potassium dichromate. The grow-up gravid females are separeted into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. At handling a care should be taken of not to penetrate the air under animals shell.

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20±2°C with variation of ±1°Cat each individual test

pH:

pH of solutions - full semi-static test: 7.7 - 8.1

Dissolved oxygen:

higher than 3 mg/L
Concentration of dissolved oxygen - full semi-static test: 7.3 - 9.2 mg/L at 19.6 - 19.9°C

Details on test conditions:

Number of test animal: 20 for each concentration and control in preliminary test
20 for each concentration and control in full semi-static test
Lighting: natural variation of daily light and dark
Without feeding, without aeration

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

1.46 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: 95% CL

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.21 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: 95% CL

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

< 0.82 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

< 0.82 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 1.47 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Effect concentrations 6

Duration:

48

Dose descriptor:

EC100

Effect conc.:

> 1.47 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

dissolved

Basis for effect:

mobility

Results with reference substance (positive control):

verification test was carried out in period from 11.12.2007 to 13.12.2007
results:
24h - EC50=1.15 mg/L (95% CL)
48h - EC50= 0.80 mg/L (95% CL)
The result of last interlaboratory test arrangment by Centre for Assessment of Laboratories of T.G.Masaryk Water Research Institute Prague in 2006 for potassium dichromate was:
48-hour EC50= 0.61 - 1.11 mg/L
The immobilisation of test species in reference substance solution expressed as EC50 fits into the accepted range in the interlaboratory tests.

Any other information on results incl. tables

Full semi-static test, Immobilisation of daphnia

Determined conc. -TWM	mg/L	1.47	1.32	1.20	1.07	0.96	0.91	0.82	C
saturated solution dosing	mL/L	1000	870	760	660	570	500	430	C
number of live animals	24 h/1st series	8	5	5	10	10	8	10	10
number of live animals	24 h/2nd series	5	4	9	7	10	10	9	10
number of live animals	24 h/ total	13	9	14	17	20	18	19	20
Immobilisation %	24 hours	35	55	30	15	0	10	5	0
number of live animals	48 h/1st series	4	3	4	7	10	8	9	10
number of live animals	48 h/2nd series	3	3	5	6	8	8	9	10
number of live animals	48 hours/total	7	6	9	13	18	16	18	20
Immobilisation %	48 hours	65	70	55	35	10	20	10	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

except point 5