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EC number: 201-126-0 | CAS number: 78-59-1
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to guideline without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Method: other: corresponding to OECD guideline 305 C
- GLP compliance:
- not specified
- Details on sampling:
- Frequency of analysis:
water: twice a week
fish: every two weeks - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- For the preparation of the stock solution suitable solvents and surfactants were used (EC50 -values of these compounds are presented)
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Fish were supplied from Sugishima fish farm
- Fish were kept in an acclimatisation tank for 1-2 months at a temperature of 25 +/- °C after external desinfection in an aqueous solution
containing 50 mg/L Terramycin and 7 g/L sodium chloride - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 42 d
- Test temperature:
- 25 °C
- Dissolved oxygen:
- 6-8 mg/l;
- Details on test conditions:
- ACCLIMATISATION:
- fish were kept in tank at a temperature of 25 +/- 2°C for ca. 28 days
- After this period, the fish were transferred into test tanks
TEST SYSTEM
1) Test tank: glass tank, water volume 100 L
2) Number of fish/level: 15-20
3) Duration of exposure: 6-8 weeks
4) Flow-through system (flow rate 200-800 ml/min, dilution water = groundwater from the test site)
5) Feeding during test: pelleted feed for carps - Nominal and measured concentrations:
- 0.5 mg/l
- Type:
- BCF
- Value:
- > 1.1 - <= 1.8 dimensionless
- Basis:
- whole body w.w.
- Remarks on result:
- other: Conc.in environment / dose:0.5 mg/L
- Type:
- BCF
- Value:
- < 10 dimensionless
- Basis:
- whole body w.w.
- Remarks on result:
- other: Conc.in environment / dose:0.05 mg/L
- Elimination:
- not specified
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Method: other: Flow-through Bioaccumulation Test
- GLP compliance:
- not specified
- Radiolabelling:
- yes
- Details on sampling:
- - samples of 5 ml water and 5 fish each on days 1, 2, 4, 7, 10, 14 (other substances: also 21 and 28) of exposure; control water on days 0, 28;
- samples of 5 fish on days 1, 2, 4, and 7 of depuration; - Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- - 50 ul (out of 50 ml) of stock solution thoroughly mixed with 500 ml water before introduction into aquarium
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- bluegill sunfish (Lepomis macrochirus)
- source: commercial fish farm in Connecticut
- wet weight 0.37 +/- 0.18 to 0.94 +/- 0.34 g;
- length 25 +/- 3 to 32 +/- 4 mm;
- acclimation period 30 days - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 14 d
- Total depuration duration:
- 7 d
- Hardness:
- total hardness: 35 mg/l as CaCO3
- Test temperature:
- 16 +/-1°C
- pH:
- 7.1
- Details on test conditions:
- TEST EQUIPMENT
- well water, pH 7.1,
- glass aquaria of 40 cm length, 20 cm width, 25 cm height / 19 cm depth;
- turnover rate six to seven aquarium volumes per day;
- temperature 16 +/- 1 °C;
- no aeration; regular determination of dissolved oxygen;
TEST PROCEDURE
- 100 fish per aquarium
- dry pelleted food ad libitum on alternating days
- removal of feces as deemed necessary
- duration until equilibrium, maximum 28 days (isophorone: 14 days),
followed by:
- transfer to aquarium with pollutant-free water;
- further duration 7 days - Nominal and measured concentrations:
- 92.4 µg/l +/- 10.5 µg/l
- Type:
- BCF
- Value:
- 7 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 14 d
- Calculation basis:
- steady state
- Elimination:
- yes
- Parameter:
- DT50
- Depuration time (DT):
- 1 d
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results indicate that isophorone has a very low potential for bioaccumulation.
- Executive summary:
The bioaccumulation potential of Isophorone was evaluated in a bioaccumulation study with bluegill sunfish (Lepomis macrochirus) according to accepted scientific principles. The BCF after 14 days of exposure was calculated to be 7 indicating that Isophorone has a very low bioaccumulation potential. The halflife which was derived from a 7-day depuration phase was calculated to be <1 day.
The reliability of the study was assessed as "reliable with restrictions":
BCF: 7
DT50: >1 day
Referenceopen allclose all
Half-life in tissues = 1 day
Description of key information
The bioconcentration factor was calculated from a flow -through fish test that was conducted with 14C labelled substance. The exposure concentration was 92.4 µg/l for 14 days with a subsequent depuration phase of 7 days (Veith, 1980).
Key value for chemical safety assessment
- BCF (aquatic species):
- 7 dimensionless
Additional information
The bioaccumulation potential of the substance was evaluated in a bioaccumulation study with bluegill sunfish (Lepomis macrochirus) according to accepted scientific principles. The BCF after 14 days of exposure was calculated to be 7 indicating that the substance has a very low bioaccumulation potential. The half-life which was derived from a 7-day depuration phase was calculated to be <1 day (Veith 1980). The low potential for bioaccumulation was also demonstrated in an OECD 305 study (with limited documentation) with Cyprinus carpio which reveals a BCF of <10 (CITI, 1992).
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