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EC number: 201-126-0 | CAS number: 78-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- study is part of a 13-week study (May 1979 - August 1979) and a 2-year carcinogenesis study (January 1980 - January 1982)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests: II. results from the testing of 270 chemicals
- Author:
- Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B and Zeiger E
- Year:
- 1 986
- Bibliographic source:
- Environ. Mutagen. Suppl. 8, 1-119
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
- Principles of method if other than guideline:
- Method: see: Haworth S, Lawlor T, Mortelmans K, Speck W and Zeiger E (1983). Salmonella mutagenicity test results for 250 chemicals. Environ
Mutagen. 5 (Suppl. 1), 3 -142. - GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Details on test material:
- other TS: Origin: Leidy Chemical Corporation 97 % pure, 0.3 % water
Constituent 1
Method
- Target gene:
- mutated gene loci responsible for histidine auxotrophy
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537
- Details on mammalian cell type (if applicable):
- Species/cell type: from Dr. B. Ames, Univ. of California
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat and hamster liver fractions
- Test concentrations with justification for top dose:
- 33, 100, 333, 1000, 3333 and 10000 (TA 1535), 100, 333, 1000, 3333, 10000 (TA 100), 33, 100, 333, 1000, 3333 (TA 98 and 1537) µg/plate
- Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Remarks:
- for details see below
- Positive control substance:
- other: for details see below
- Remarks:
- no remarks
- Details on test system and experimental conditions:
- Type: Ames test
ADMINISTRATION:
- Number of replicates: 3 per dose level, repeated
- Positive and negative control groups and treatment:
sodium azide positive for TA 1535 and TA 100, 4-nitro-o-phenylenediamine positive for TA 98, 9-aminoacridine positive for TA 97 and TA 1537,
2-aminoanthracene positive all strains, potassium chloride negative
- Solvent: H2O
- 3 investigations were performed: 1. without MA, 2. with MA (Arochlor-1254 liver rats) 3. with MA (Arochlor-1254 liver hamster)
Cells and test compound or solvent (water) were incubated for 20 minutes at 37 °C in the presence of either S9 or buffer (0.1 M PO4, pH 7.4).
After the addition of soft agar, the contents of each tube were poured onto 25 ml of minimal glucose bottom agar. When the top agar had solidified,
the plates were inverted and incubated at 37 °C for 48 hours (Haworth et al. 1983). The analysis was performed twice, each in triplicate. - Evaluation criteria:
- 1. mutagenic response: a dose-related, reproducible increase in the number of revertants over background, even if the increase was less than
twofold
2. nonmutagenic response: when no increase in the number of revertants was elicited by the chemical
3. questionable response: when there was an absence of a clear-cut-dose-related increase in revertants; when the dose-related increases in the
number of revertants were not reproducible; or when the response was of insufficient magnitude to support a determination of mutagenicity. - Statistics:
- not reported
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: -S9: TA 100, 1535, 98 >=1000, TA 1537 >= 3333 µg/plate, +S9 (rat and hamster): only TA 1535 = 10000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- GENTOXIC EFFECTS:
- With metabolic activation: None (even at cytotoxic concentrations)
- Without metabolic activation: None (even at cyctotoxic concentrations)
CYTOTOXIC CONCENTRATION:
TA 98: >= 1000 µg/plate (-S9)
TA 100: >= 1000 µg/plate (-S9)
TA 1535: >= 1000 µg/plate (-S9); 1000 µg/plate (+S9)
TA 1537: >= 3333 µg/plate (-S9)
- Remarks on result:
- other: other: Salmonella typhimurium TA98, TA100, TA1535, TA1537
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
no remarks
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this study, Isophorone was not mutagenic in strains TA 100, TA 1535, TA 1537, or TA 98 of Salmonella typhimurium in the
presence or absence of Aroclor 1254-induced Sprague-Dawley male rat or male Syrian hamster liver S9. - Executive summary:
The test substance Isophorone was tested for any mutagenic activity in the Ames Salmonella/microsomes test. Four histidine-
auxotrophic Salmonella typhimurium strains TA 98, TA 100, TA 1535, and TA1537 were treated with the test item by the pre-incubation method. Dose levels covering a total range of 33 to 10000 µg/plate, in triplicate both with and without the addition of a metabolising system (Aroclor induced rat and hamster liver S9 mix) were employed.
A mutagenic activity of Isophorone to any of the five tester strains was not observed with and without metabolic activation. It is therefore concluded, that Isophorone is not a bacterial mutagen.
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