Glycerides, C16-22

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

Not available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to OECD Guideline 203. No analytical verification of test concentrations.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: 5.1.1 "Acute toxicity for Fish" of the EEC-Directive 79/831

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Glycerides, C16-C18 and C18 unsatd.

IUPAC Name:

Glycerides, C16-C18 and C18 unsatd.

Details on test material:

- Name of test material (as cited in study report): Alkali-refined linseed oil (CAS N° 8001-26-1, EC N° 232-278-6). Under the SDA nomenclature, the name of this substance is 'Glycerides, C16-C18 and C18 unsatd.'
- Substance type: Triglycerdes of vegetable origin

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
None

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

No data

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: Barachydanio rerio (Teleostei, Cyprinidae)
- Source: West Aquarium GmbH
- Age at study initiation (mean and range, SD): 2 months


ACCLIMATION
- Acclimation conditions: The fish were held together in a glass tank (200 L). Reconstituted water was used as holding water (according to OECD-guideline 203)
- Type and amount of food: Tetra Min(R) Hauptfutter (Art. no. 1004 DN, produced by Tetra-Werke). The amount was regulated according to the actual consumption observed
- Feeding frequency: Twice daily
- Health during acclimation (any mortality observed): All were in good health and condition, no mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

No data

Test temperature:

Between 21 and 25 °C

pH:

6.0 to 8.5

Dissolved oxygen:

>60%

Salinity:

No data

Nominal and measured concentrations:

100 and 1,000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: length 300 mm, with 200 mm and height 220 mm
- Aeration: an aquarium aerators, brand Optima (Art. no. A 10007, produced by Weltweit GmbH & Co. KG, D- 2081) were used for continuous aeration
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Details on results:

- Mortality of control: no deaths
- Other adverse effects control: none
- Abnormal responses: no

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

None

Any other information on results incl. tables

Sublethal observations / clinical signs:

None

Applicant's summary and conclusion

Conclusions:

Under the test conditions, the 96 h LC0 (Brachydanio rerio) for linseed oil was found to be 1,000 mg/L (based on nominal concentrations).

Executive summary:

A study was conducted to evaluate lethal effects of linseed oil to Zebra fish (Brachydanio rerio). The test was carried out according to OECD Guideline 203.

Fish were exposed in separate test batches to different concentration (100 and 1,000 mg/L) of the test material in the test medium over a test period of 96 h (each tank with 10 fish). The observations were made at 0, 24, 48, 72 and 96 h for mortality , abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed.

Under the test conditions, the 96 h LC0 (Brachydanio rerio) for linseed oil was found to be 1,000 mg/L (based on nominal concentrations).